DRAVP ID  DRAVPe01858

Peptide Name   Entry 26

Sequence  rRRKSXSf

Sequence Length  8

UniProt ID  No entry found

Taxon ID  None

Source  Synthetic construct

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  DENV

Assay  Fluorimetric assay

Activity 

• [Ref.28539222]dengue virus 2(DENV2):inhibition of the activity of NS2B-NS3 protease(IC50= 3.7 μM); inhibition of virus-induced cytopathic effects (CPE) in BHK-21 cells(EC50=87.5 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.28539222]BHK-21 cells: CC50>400 μM.

Binding Target  NS2B-NS3 protease

Mechanism  The cyclic peptide could inhibit the activity of NS2B-NS3 protease, which is an essential enzyme for the replication of dengue virus.

PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Cyclic

N-terminal Modification  No specific N-terminal

C-terminal Modification  No specific C-terminal

Other Modification  The 'X' at position 6 indicates biphenylalanine (L-bPhe). The N-terminal and C-terminal cyclized by a amide bond.

Stereochemistry  Mixed(D-Arg1, Phe8)

Literature 1

Title   Discovery of novel cyclic peptide inhibitors of dengue virus NS2B-NS3 protease with antiviral activity.

Pubmed ID   28539222

Reference   Bioorg Med Chem Lett. 2017 Aug 1;27(15):3586-3590.

Author   Takagi Y, Matsui K, Nobori H, Maeda H, Sato A, Kurosu T, Orba Y, Sawa H, Hattori K, Higashino K, Numata Y, Yoshida Y.

DOI   10.1016/j.bmcl.2017.05.027