General Information

General Information

DRAVP ID DRAVPe01947

Peptide Name TAT-Kα2

Sequence RRRQRRKKRGYGFVNLLFLVVE

Sequence Length 22

UniProt ID No entry found

Source Synthetic construct

Activity Information

Target Organism VSV,NDV,RSV,Influenza virus, HSV

Assay Plaque assay

Activity

[Ref.28687749]Vesicular stomatitis virus(VSV):inhibition of viral infection in 293T cells(IC50=2.9 μM);
Respiratory Syncytial Virus(RSV):inhibition of viral infection in Hep2 cells(IC50=3.4 μM);
Newcastle disease virus(NDV):inhibition of viral infection(IC50=0.8 μM);
Parainfluenza virus:inhibition of viral infection(IC50=2.4 μM);
Influenza virus (H1N1) A/Puerto Rico/8/34:inhibition of viral infection(IC50=0.5 μM);
Influenza virus (H5N2) A/bird/Korea/W81/2005:inhibition of viral infection(IC50=0.8 μM);
Influenza virus (H7N3) A/Aquatic bird/Korea/W44/2005:inhibition of viral infection(IC50=1.2 μM);
Influenza virus (H9N2) A/Chicken/Korea/116/2004:inhibition of viral infection(IC50=0.7 μM);
Porcine epidemic diarrhea virus:inhibition of viral infection(IC50=3.5 μM);
Herpes simplex virus:inhibition of viral infection(IC50=5.7 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.28687749]No cytotoxicity against MDCK cells up to 100 μM.

Binding Target Envolope

Mechanism display virucidal activity by disrupting viral envelopes.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C126H212N44O28

Absent amino acids ACDHIMPSTW

Common amino acids R

Mass 2791.35

Pl 12.01

Basic residues 8

Acidic residues 1

Hydrophobic residues 8

Net charge 7

Boman Index -8503

Hydrophobicity -80.91

Aliphatic Index 92.73

Half Life

Mammalian:1 hour
Yeast:2 min
E.coli:2 min

Extinction Coefficient cystines 1490

Absorbance 280nm 70.95

Polar residues 4

Literature Information

Literature 1

Title Inhibition of highly pathogenic avian influenza (HPAI) virus by a peptide derived from vFLIP through its direct destabilization of viruses

Pubmed ID 28687749

Reference Sci Rep. 2017 Jul 7;7(1):4875.

Author Moon HJ, Nikapitiya C, Lee HC, Park ME, Kim JH, Kim TH, Yoon JE, Cho WK, Ma JY, Kim CJ, Jung JU, Lee JS.

DOI 10.1038/s41598-017-04777-4