General Information

General Information

DRAVP ID DRAVPe02095

Peptide Name Ala-6-fenycin

Sequence EKYTEAPEYI

Sequence Length 10

UniProt ID None

Taxon ID None

Source Bacillus sp. strain P34

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism EAV, FHV-1

Assay Antiviral assay.

Activity

[Ref:25477947]Equine arteritis virus (EAV): P34 peptide inhibited replication by 99.9%, and Feline herpesvirus-1 (FHV-1) by 94.4%.

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref:25477947]MDCK cells: CC50 = 2.11 µg/mL,CRFK cells: CC50 = 2.5 µg/mL,RK13 cells: CC50 = 3.92 µg/mL,MDCK cells: No cytotoxicity observed at 1.37 µg/mL of peptide P34,CRFK cells: No cytotoxicity obs

Binding Target Membrane

Mechanism This peptide inactivates the virus through an interaction with a non-lipidic structural component.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Carboxylation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C57H83N11O20

Absent amino acids RNDCQLMFSWVOU

Common amino acids E

Mass 1242.35

Pl 4.25

Basic residues 1

Acidic residues 3

Hydrophobic residues 5

Net charge -2

Boman Index 2.21

Hydrophobicity -1.3

Aliphatic Index 49

Half Life

Mammalian:1 hour
Yeast:30 min
E.coli:>10 hours

Extinction Coefficient cystines 2980

Absorbance 280nm 2.399

Polar residues 5

Literature Information

Literature 1

Title Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals

Pubmed ID 25477947

Reference Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 45(3), 1089–1094

Author Scopel e Silva, D., de Castro, C. C., da Silva e Silva, F., Sant'anna, V., Vargas, G. D., de Lima, M., Fischer, G., Brandelli, A., da Motta, A.deS., & Hübner, S.deO. (2014)

DOI 10.1590/s1517-83822014000300043

Literature 2

Title Antimicrobial Peptides: An Emerging Category of Therapeutic Agents

Pubmed ID 28083516

Reference Frontiers in cellular and infection microbiology, 6, 194

Author Mahlapuu, M., Håkansson, J., Ringstad, L., & Björn, C. (2016)

DOI 10.3389/fcimb.2016.00194