DRAVP ID  DRAVPe02118

Peptide Name   Gramicidin A

Sequence  VGALAVVVWLWLWLW

Sequence Length  15

UniProt ID  None  

Taxon ID  None 

Source  Soil bacterium, Bacillus brevis

Validation   Experimentally validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Found

Target Organism  HIV,HSV

Assay  ELISA

Activity 

• [Ref:9672588]HSV-1,HSV-2: IC50=0.2–0.4 mg/ml (HSV-1), 0.1–0.3 mg/ml (HSV-2)

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  Not Found

Mechanism  gramicidin suppressed the replication of ACV-resistant thymidine kinase and DNA polymerase HSV mutants at doses effective against ACV-sensitive strains.

PDB ID  1MAG 

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Synthesized non-ribosomally by a multienzyme complex. Both the N- and C-termini are chemically modified (CHO at the N-terminus and NHCH2CH2OH at the C-terminus). N-terminus is rich in Val, while the C-terminus is rich in Trp. Amino acids 4, 6, 8, 10, and 12 are D-amino acids.

Stereochemistry  L

Literature 1

Title   The effect of gramicidin, a topical contraceptive and antimicrobial agent with anti-HIV activity, against herpes simplex viruses type 1 and 2 in vitro

Pubmed ID   9672588

Reference   Archives of virology, 142(11), 2225–2235.

Author   Bourinbaiar, A. S., & Coleman, C. F. (1997).

DOI   10.1007/s007050050237