General Information

General Information

DRAVP ID DRAVPe02144

Peptide Name Pa-MAP

Sequence HTASDAAAAAALTAANAAAAAAASMA

Sequence Length 26

UniProt ID None

Taxon ID None

Source polar fish Pleuronectes americanus

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HSV-1

Assay Anti-HSV-Assay

Activity

[Ref:27161201]HSV-1:IC50=82%(45μM),HSV-1:IC50=90%(23μM)

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref:27161201]Pa-MAP did not reach the concentrations that allowed 50% of cellular viability (CC50), since there were already 80% of viable cells in the first dilution. Even though some peptides showe

Binding Target Membrane

Mechanism Pa-MAP could possible interact with the viral envelope and the phospholipids of the viral surface, due to the presence of hydrophobic amino acid residues.22 According to such data, further tests were performed in this study to define Pa-MAP’s antiviral mechanism of action. It was verified that this peptide has a virucidal action,like most antiviral peptides described in the literature,13 inhibiting the virus’ action before adsorption, at a percentage above 80%, reinforcing the theory presented by Migliolo et al.,22 and the SI was higher than 5 considering the CC50 at 400 mg.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Cyclic

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C90H149N29O34S1

Absent amino acids RCQEGIKFPWYVOU

Common amino acids A

Mass 2213.41

Pl 5.08

Basic residues 0

Acidic residues 1

Hydrophobic residues 18

Net charge -1

Boman Index 0.9185

Hydrophobicity 0.888

Aliphatic Index 80.38

Half Life

Mammalian:3.5 hours
Yeast:10 min
E.coli:>10 hours

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 22

Literature Information

Literature 1

Title Linear antimicrobial peptides with activity against herpes simplex virus 1 and Aichi virus

Pubmed ID 27161201

Reference Biopolymers, 108(2), 10.1002/bip.22871.

Author Vilas Boas, L. C., de Lima, L. M., Migliolo, L., Mendes, G. D., de Jesus, M. G., Franco, O. L., & Silva, P. A. (2017).

DOI 10.1002/bip.22871