General Information

General Information

DRAVP ID DRAVPe02224

Peptide Name Tricyclic peptide RP 71955

Sequence CLGIGSCNDFAGCGYAVVCFW

Sequence Length 21

UniProt ID P37046

Taxon ID 72594

Source Streptomyces sp. (strain SP9440) (Gram-positive bacteria)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position 1 to 21

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism HIV-1

Assay Antiviral Assay

Activity

[Ref:8286361] inhibited the HIV-1 aspartyl protease (IC50, 35 ug/ml) a

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target Not found

Mechanism Not Available

Structure Information

PDB ID 1RPB 1RPC

Predicted Structure Download

Linear/Cyclic Cyclic

N-terminal Modification Cyclization of a N-terminal between Cys1 and Asp9

C-terminal Modification Free

Other Modification An internal amide bond between the NH2 of C1 and the gamma-COOH of D9 was observed, as well as two disulfide bridges, one between C1 and C13 and one between C7 and C19. In contrast. Class 2 lassos do not have disulfide bonds.

Stereochemistry L

Physicochemical Information

Formula C97H137N23O27S4

Absent amino acids EHKMPQRT

Common amino acids CG

Mass 2185.53

Pl 3.8

Basic residues 0

Acidic residues 1

Hydrophobic residues 9

Net charge -1

Boman Index 19.81

Hydrophobicity 1.157

Aliphatic Index 74.29

Half Life

Mammalian:1.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 7240

Absorbance 280nm 362

Polar residues 11

Literature Information

Literature 1

Title Solution structure of RP 71955, a new 21 amino acid tricyclic peptide active against HIV-1 virus;

Pubmed ID 8286361;

Reference Biochemistry. 1994 Jan 11;33(1):42-50;

Author Fréchet D, Guitton JD, Herman F, Faucher D, Helynck G, Monegier du Sorbier B, Ridoux JP, James-Surcouf E, Vuilhorgne M.

DOI 10.1021/bi00167a006;

Literature 2

Title Isolation of RP 71955, a new anti-HIV-1 peptide secondary metabolite.

Pubmed ID 8270499

Reference J Antibiot (Tokyo). 1993 Nov;46(11):1756-1757.

Author

DOI 10.7164/antibiotics.46.1756