To the extent possible under law, the person who associated CC0 with this work has waived all copyright and related or neighboring rights to this work.
General Information
DRAVP ID DRAVPe02246
Peptide Name 7-1 peptide
Sequence LVRPLAL
Sequence Length 7
UniProt ID No entry found
Taxon ID None
Source Synthetic construct
Validation Experimentally Validated
Origin Information
Gene Name/ID Not Available
GenBank Not Available
Amino Acid position Not Available
Domain Accession ID Not Available
Nucleotide sequence ID Not Available
Molecular Type Not Available
Chromosomal Position Not Available
Activity Information
Target Organism Influenza A H3N2, A/Aichi/2/68 (H3N2)
Assay Plaque reduction
Activity
Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry
Cytotoxicity
Binding Target Head of HA
Mechanism A random phage display library of 7-mer peptides was screened by affinity selection, obtaining the peptide LVRPLAL that showed the highest affinity toward H1 and H3 HAs. The avidin–biotin-peroxidase complex (ABC) system was exploited to evaluate the binding of this peptide to HA. It showed a Kd of 92 and 194 µM for H1 HA from A/New Caledonia/20/99 (H1N1) and H3 from A/Wyoming/3/2003 (H3N2), respectively. The C18-conjugated form of this peptide was tested in plaque reduction assay, showing a good activity toward A/Aichi/2/68 (H3N2) virus (6.4 µM) and a lower potency against A/Puerto Rico/8/34 (H1N1) virus (101 µM). The Ala scan indicated the importance of Arg3 and Pro4 for the HA binding, which was also assessed by docking calculations
Structure Information
PDB ID None
Linear/Cyclic Linear
N-terminal Modification Not included yet
C-terminal Modification Not included yet
Other Modification None
Stereochemistry L
Physicochemical Information
Formula C37H68N10O8
Absent amino acids NDCQEGHIKMFSTWYOU
Common amino acids L
Mass 781.01
Pl 9.79
Basic residues 1
Acidic residues 0
Hydrophobic residues 5
Net charge -1
Boman Index -0.81
Hydrophobicity 1.614
Aliphatic Index 222.86
Half Life
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 0
Literature Information
Literature 1
Title Heptapeptide ligands against receptor-binding sites of influenza hemagglutinin toward anti-influenza therapy
Pubmed ID 26833245
Reference Bioorganic & medicinal chemistry, 24(5), 1106–1114.
Author Matsubara, T., Onishi, A., Yamaguchi, D., & Sato, T. (2016).
To the extent possible under law, the person who associated CC0 with this work has waived all copyright and related or neighboring rights to this work.