DRAVP ID  DRAVPe02271

Peptide Name   Labyrinthopeptin A1

Sequence  SNASVWECCSTGSWVPFTCC

Sequence Length  20

UniProt ID  C0MP59 

Taxon ID  182080 

Source  Actinomycete Actinomadura namibiensis DSM 6313

Validation   Experimentally Validated

Gene Name/ID  labA1

GenBank  Not Available

Amino Acid position  21-40

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  DENV,ZIKV,WNV,HCV,CHIKV,KSHV,CMV,HSV-2,HIV-1

Assay  Antiviral Assay

Activity 

• [Ref:3166638]CHIKV IC₅₀ = 2.2 μM,CHIKV (pseudotype):IC₅₀ = 1.8 μM,DENV-1 IC₅₀ = 0.8 μM,DENV-2 (Huh-7, high content imaging):IC₅₀ = 1.8 μM,DENV-2 (Huh-7, qRT-PCR) :IC₅₀ = 2.0 μM,DENV-2 (MDDC, flow cytometry): IC₅₀ = 0.4 μM,DENV-2 (MDDC, qRT-PCR): IC₅₀ = 0.3 μM,DENV-3 IC₅₀ = 1.1 μM,DENV-4: IC₅₀ = 0.3 μM,HCMV:IC₅₀ = 1.3 μM,HCV:IC₅₀ = 1.1 μM,HIV (laboratory strain, MT-4 T cells);IC₅₀ = 1.9 μM,HIV (laboratory strain, PBMC):IC₅₀ = 1.7 μM,HIV (laboratory strain, MDM):IC₅₀ = 2.4 μM,HIV (clinical isolate, PBMC):IC₅₀ = 1.0 μM,HSV-1:IC₅₀ = 1.6 μM,HSV-1 (Tk-deficient):IC₅₀ = 1.9 μM,HSV-2:IC₅₀ = 0.4 μM,HSV-2 (Tk-deficient):IC₅₀ = 0.3 μM,KSHV:IC₅₀ = 2.0 μM,TBEV:IC₅₀ = 24.1 μM,WNV:IC₅₀ = 0.2 μM,ZIKV-976 (Huh-7, high content imaging):IC₅₀ = 2.0 μM,ZIKV-976 (Huh-7, qRT-PCR):IC₅₀ = 1.8 μM,ZIKV-H/PF-2013 (Huh-7, high content imaging):IC₅₀ = 1.6 μM,ZIKV-H/PF-2013 (Huh-7, qRT-PCR):IC₅₀ = 1.6 μM.

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref:3166638]Labyrinthopeptins exhibited low cytotoxicity and had favorable pharmacokinetic properties in mice (half-life [t1/2] = 10.0 h),

Binding Target  Membrane

Mechanism  LabyA1 exert the antiviral function by disrupting viral envelopes (virolysis).

PDB ID  None

Predicted Structure Download 

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   Labyrinthopeptins Exert Broad-Spectrum Antiviral Activity through Lipid-Binding-Mediated Virolysis

Pubmed ID   31666384

Reference   Journal of virology, 94(2), e01471-19

Author   Prochnow, H., Rox, K., Birudukota, N. V. S., Weichert, L., Hotop, S. K., Klahn, P., Mohr, K., Franz, S., Banda, D. H., Blockus, S., Schreiber, J., Haid, S., Oeyen, M., Martinez, J. P., Süssmuth, R. D., Wink, J., Meyerhans, A., Goffinet, C., Messerle, M., Schulz, T. F., … Brönstrup, M. (2020)

DOI   10.1128/JVI.01471-19

Literature 2

Title   The lantibiotic peptide labyrinthopeptin A1 demonstrates broad anti-HIV and anti-HSV activity with potential for microbicidal applications

Pubmed ID   23724015

Reference   PloS one, 8(5), e64010.

Author   Férir, G., Petrova, M. I., Andrei, G., Huskens, D., Hoorelbeke, B., Snoeck, R., Vanderleyden, J., Balzarini, J., Bartoschek, S., Brönstrup, M., Süssmuth, R. D., & Schols, D. (2013).

DOI   10.1371/journal.pone.0064010