General Information

General Information

DRAVP ID DRAVPe02272

Peptide Name Labyrinthopeptin A2

Sequence SDWSLWECCSTGSLFACC

Sequence Length 18

UniProt ID C0MP60

Taxon ID 182080

Source A.namibiensis

Validation Experimentally Validated

Origin Information

Gene Name/ID labA2

GenBank Not Available

Amino Acid position 21-38

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism DENV,ZIKV,WNV,HCV,CHIKV,KSHV,CMV,HSV

Assay Antiviral Assay

Activity

[Ref:3166638]CHIKV (pseudotype) IC₅₀ = >5.0 μM,DENV-1 IC₅₀ = 12.0 μM,DENV-2 (Huh-7, high content imaging) IC₅₀ = 8.0 μM,DENV-2 (Huh-7, qRT-PCR) IC₅₀ = 7.6 μM,DENV-2 (MDDC, flow cytometry) IC₅₀ = 5.2 μM,DENV-2 (MDDC, qRT-PCR) IC₅₀ = 4.2 μM,DENV-3 IC₅₀ = 9.4 μM,DENV-4 IC₅₀ = 4.0 μM,HCMV IC₅₀ = 5.4 μM,HCV IC₅₀ = 1.7 μM,HSV-1 IC₅₀ = 12.4 μM,HSV-1 (Tk-deficient) IC₅₀ = 10.7 μM,HSV-2 IC₅₀ = 5.5 μM,HSV-2 (Tk-deficient) IC₅₀ = 3.2 μM,KSHV IC₅₀ = 15.0 μM,TBEV IC₅₀ = >26.0 μM,WNV IC₅₀ = 0.7 μM,ZIKV-976 (Huh-7, high content imaging) IC₅₀ = 9.6 μM,ZIKV-976 (Huh-7, qRT-PCR) IC₅₀ = 5.7 μM,ZIKV-H/PF-2013 (Huh-7, high content imaging) IC₅₀ = 3.3 μM,ZIKV-H/PF-2013 (Huh-7, qRT-PCR) IC₅₀ = 3.4 μM.

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref:3166638]Labyrinthopeptins exhibited low cytotoxicity and had favorable pharmacokinetic properties in mice (half-life [t1/2] = 10.0 h),

Binding Target Membrane

Mechanism LabyA2 exert the antiviral function by disrupting viral envelopes (virolysis).

Structure Information

PDB ID None

Predicted Structure Download

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C85H120N20O28S4

Absent amino acids RNQHIKMPYVOU

Common amino acids CS

Mass 1998.24

Pl 3.67

Basic residues 0

Acidic residues 2

Hydrophobic residues 6

Net charge -2

Boman Index 0.35

Hydrophobicity 0.506

Aliphatic Index 48.89

Half Life

Mammalian:1.9hours
Yeast:>20hours
E.coli:>10hours

Extinction Coefficient cystines 11250

Absorbance 280nm 5.63

Polar residues 10

Literature Information

Literature 1

Title Labyrinthopeptins Exert Broad-Spectrum Antiviral Activity through Lipid-Binding-Mediated Virolysis

Pubmed ID 31666384

Reference Journal of virology, 94(2), e01471-19

Author Prochnow, H., Rox, K., Birudukota, N. V. S., Weichert, L., Hotop, S. K., Klahn, P., Mohr, K., Franz, S., Banda, D. H., Blockus, S., Schreiber, J., Haid, S., Oeyen, M., Martinez, J. P., Süssmuth, R. D., Wink, J., Meyerhans, A., Goffinet, C., Messerle, M., Schulz, T. F., … Brönstrup, M. (2020).

DOI 10.1128/JVI.01471-19