DRAVP ID  DRAVPe02273

Peptide Name   Latarcin 1

Sequence  SMWSGMWRRKLKKLRNALKKKLKGE

Sequence Length  25

UniProt ID  Q1ELT9 

Taxon ID  379576 

Source  Lachesana tarabaevi

Validation   Experimentally Validated

Gene Name/ID  Lt1a

GenBank  Not Available

Amino Acid position  63-87

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  DENV

Assay  Dengue NS2B-NS3 protease Assay

Activity 

• [Ref:24885331]Dengue protease NS2B-NS3pro (37°C) IC₅₀ = 12.68 ± 3.2 μM,Dengue protease NS2B-NS3pro (40°C) IC₅₀ = 6.58 ± 4.1 μM,Reduction in viral RNA (24 h) EC₅₀ = 8.3 ± 1.2 μM,Reduction in viral RNA (48 h) EC₅₀ = 7.6 ± 2.7 μM,Reduction in viral RNA (72 h) EC₅₀ = 6.8 ± 2.5 μM.

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref:24885331]The CC50 value of the Ltc 1 peptide obtained via the optimisation steps was estimated to be approximately 52.51 ± 3.6 μM

Binding Target  NS2B-NS3

Mechanism  The Ltc 1 peptide exhibited significant inhibitory effects against dengue NS2B-NS3pro and virus replication in the infected cells

PDB ID  2PCO 

Predicted Structure Download 

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   Identification of natural antimicrobial agents to treat dengue infection: In vitro analysis of latarcin peptide activity against dengue virus

Pubmed ID   24885331

Reference   BMC microbiology, 14, 140

Author   Rothan, H. A., Bahrani, H., Rahman, N. A., & Yusof, R. (2014)

DOI   10.1186/1471-2180-14-140