DRAVP ID  DRAVPe02281

Peptide Name   Micrococcin P1

Sequence  SCTTCVCTCSCCTT

Sequence Length  14

UniProt ID  No entry found

Taxon ID  None

Source  Staphylococcus epidermidis strain 115; Staphylococcus equorum WS 2733, from a French red smear cheese

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  HCV

Assay  HCVcc inhibition assay

Activity 

• [Ref:27387825]HCV:H77 (Huh7.5 cells), EC₅₀ = 0.222 ± 0.064 μM;Jc1 (Huh7.5 cells), EC₅₀ = 0.396 ± 0.016 μM;S52 (Huh7.5 cells), EC₅₀ = 0.141 ± 0.004 μM;DE43 (Huh7.5 cells), EC₅₀ = 0.269 ± 0.002 μM;SA13 (Huh7.5 cells), EC₅₀ = 0.339 ± 0.054 μM;HK6a (Huh7.5 cells), EC₅₀ = 0.325 ± 0.027 μM;QC69 (Huh7.5 cells), EC₅₀ = 0.304 ± 0.093 μM;JFH1-GFP (Huh7.5 cells), EC₅₀ = 0.80 ± 0.28 μM;Jc1 Renilla (Huh7.5 cells), EC₅₀ = 0.52 ± 0.08 μM; TN NanoLuc (Huh7.5 cells), EC₅₀ = 0.54 ± 0.01 μM

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref:27387825]CC50 was 11.5 ± 0.14 μM

Binding Target  Membrane

Mechanism  Micrococcin P1 strongly inhibits HCV attachment similar to anti-CD81 antibodies. however, its effect was shown prior to fusion of the viral envelope to the endosomal membrane

PDB ID  None

Predicted Structure Download 

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   Micrococcin P1, a naturally occurring macrocyclic peptide inhibiting hepatitis C virus entry in a pan-genotypic manner

Pubmed ID   27387825

Reference   Antiviral research, 132, 287–295.

Author   Lee, M., Yang, J., Park, S., Jo, E., Kim, H. Y., Bae, Y. S., & Windisch, M. P. (2016).

DOI   10.1016/j.antiviral.2016.07.002