General Information

General Information

DRAVP ID DRAVPe02284

Peptide Name ModoCath5

Sequence WYQLIRTFGNLIHQKYRKLLEAYRKLRD

Sequence Length 28

UniProt ID F6W4B4

Taxon ID 13616

Source The gray short-tailed opossum, Monodelphis domestica, South America

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Found

GenBank Not Found

Amino Acid position 133-160

Domain Accession ID Not Available

Nucleotide sequence ID CM000373.1

Molecular Type Not Found

Chromosomal Position Primary_assembly 6: 195,029,250-195,034,644 reverse strand.

Activity Information

Target Organism WNV

Assay Antiviral Assay

Activity

[Ref:32194564]ΔModoCath5, resulting in approximately 500-fold decrease in virus production

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref:32194564]CC₅₀:HEK293T: 93.7% (8 µg/mL), 67.4% (16 µg/mL), 47.8% (32 µg/mL), 35.0% (64 µg/mL),Human primary keratinocytes: 98.1% (8 µg/mL), 96.2% (16 µg/mL), 79.1% (32 µg/mL), 48.9% (64 µg/mL),MCF

Binding Target Not Found

Mechanism The presence of ΔModoCath5 resulted in lower CXCL10, IFIT2, and IL28A mRNA levels, which can be related to the reduced viral replication in keratinocytes treated with this peptide. While viperin expression was not modified, ΔModoCath5 tended to increase ISG20 expression in WNV-infected keratinocytes. However, at the protein level, type III interferon secretion in response to WNV infection was not modulated by ΔModoCath5 treatment

Structure Information

PDB ID None

Predicted Structure Download

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C169H265N49O40

Absent amino acids CMOPSUV

Common amino acids L

Mass 3623.27

Pl 10.28

Basic residues 7

Acidic residues 2

Hydrophobic residues 10

Net charge 5

Boman Index 2.66

Hydrophobicity -0.846

Aliphatic Index 101.07

Half Life

Mammalian:2.8hours
Yeast:3min
E.coli:2min

Extinction Coefficient cystines 9970

Absorbance 280nm 2.752

Polar residues 17

Literature Information

Literature 1

Title Opossum Cathelicidins Exhibit Antimicrobial Activity Against a Broad Spectrum of Pathogens Including West Nile Virus

Pubmed ID 32194564

Reference Broad Spectrum of Pathogens Including West Nile Virus. Frontiers in immunology, 11, 347.

Author Cho, H. S., Yum, J., Larivière, A., Lévêque, N., Le, Q. V. C., Ahn, B., Jeon, H., Hong, K., Soundrarajan, N., Kim, J. H., Bodet, C., & Park, C. (2020).

DOI 10.3389/fimmu.2020.00347