DRAVP ID  DRAVPe02288

Peptide Name   NP-06

Sequence  CLGVGSCNDFAGCGYAIVCFW

Sequence Length  21

UniProt ID  P85078 

Taxon ID  1931 

Source  Streptomyces strain AA6532

Validation   Experimentally Validated

Gene Name/ID  Tricyclic peptide MS-271 (protein)

GenBank 

Amino Acid position  1 to 21

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not Available

Target Organism  HIV-1

Assay  HIV cytopathic effect inhibition assay

Activity 

• [Ref:8619594]HIV-1LAI:IC50=2.8 mM,HIV-2ROD:IC50=6.0 mM,HIV-1ERS104pre:IC50=1.3 mM

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref:8619594]HIV-1LAI:CC50=28 mM,HIV-2ROD:CC50=0.23 mM,HIV-1ERS104pre:CC50=33 mM

Binding Target  Membrane

Mechanism  NP-06 appears to block the early stage of HIV-1 infection, most likely at the stage of virus-cell fusion.

PDB ID  None

Predicted Structure Download 

Linear/Cyclic  Cyclic

N-terminal Modification  Amidation

C-terminal Modification  Carboxylation

Other Modification  A disulfide bond between Cys-7 and Cys-19 was also indicated by the cross peak between Hb of Cys-7 and Hb of Cys-1.

Stereochemistry  L

Literature 1

Title   NP-06: a novel anti-human immunodeficiency virus polypeptide produced by a Streptomyces species

Pubmed ID   8619594

Reference   Chokekijchai, S., Kojima, E., Anderson, S., Nomizu, M., Tanaka, M., Machida, M., Date, T., Toyota, K., Ishida, S., & Watanabe, K. (1995). 

Author   Antimicrobial agents and chemotherapy, 39(10), 2345–2347.

DOI   10.1128/AAC.39.10.2345