DRAVP ID  DRAVPe02296

Peptide Name   NP-1

Sequence  VVCACRRALCLPRERRAGFCRIRGRIHPLCCRR

Sequence Length  33

UniProt ID  P01376 

Taxon ID  9986 

Source  Oryctolagus cuniculus (Rabbit)

Validation   Experimentally Validated

Gene Name/ID  Corticostatin-3(protein)

GenBank  AAA31387.1

Amino Acid position  63-95

Domain Accession ID  Not Available

Nucleotide sequence ID  M28883 

Molecular Type  mRNA

Chromosomal Position  Scaffold GL018713: 117,470-184,536 forward strand

Target Organism  HSV-2

Assay  Antiviral Assay

Activity 

• [Ref:12543649] HSV-2:IC50= 98.7% ± 0.7%. NP-1 inhibited ∼90% of viral infection when added at t = 0 h.reduced viral infection by only 15% ± 8.3%.NP-1 reduced the number of plaques by 59.0% ± 16.6% compared to control acetic acid buffer

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref:12543649]No cytotoxic effect was observed even after 20 h of exposure to NP-1 at the concentration of 25 or 100 μg/ml

Binding Target  Membrane

Mechanism  NP-1 followed by extensive washing still partially inhibits infection suggests that the defensin may interact with or persist locally on cell surfaces.NP-1,prevents VP16 from being translocated to the nucleus, suggesting that NP-1 blocks viral entry.

PDB ID  None

Predicted Structure Download 

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   NP-1, a rabbit alpha-defensin, prevents the entry and intercellular spread of herpes simplex virus type 2.

Pubmed ID   12543649

Reference   Antimicrobial agents and chemotherapy, 47(2), 494–500

Author   Sinha, S., Cheshenko, N., Lehrer, R. I., & Herold, B. C. (2003).

DOI   10.1128/AAC.47.2.494-500.2003