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General Information
DRAVP ID DRAVPe02310
Peptide Name 3-Chol
Sequence KKKKGSGC
Sequence Length 8
UniProt ID No entry found
Taxon ID None
Source Synthetic construct
Validation Experimentally Validated
Origin Information
Gene Name/ID Not Available
GenBank Not Available
Amino Acid position Not Available
Domain Accession ID Not Available
Nucleotide sequence ID Not Available
Molecular Type Not Available
Chromosomal Position Not Available
Activity Information
Target Organism EBOV
Assay Not Available
Activity
Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry
Cytotoxicity
Binding Target Glycoprotein.
Mechanism 3-Chol, comprising a tetralysine segment linked to cholesterol, effectively inhibits both VSV-GP and VSV-G viral entry, with over 90% inhibition at higher concentrations. Despite lacking the GP CHR sequence, its activity against VSV-GP suggests a potential dependency on the native C-heptad repeat sequence. The study underscores the non-specific inhibitory role of cholesterol-conjugated peptides like 3-Chol against VSV-GP entry, implicating a mechanism involving both peptide and cholesterol components in inhibiting Ebola virus glycoprotein-mediated cell entry.
Structure Information
PDB ID None
Linear/Cyclic Linear
N-terminal Modification cholesterylation
C-terminal Modification Free
Other Modification In the peptide sequence, the cysteine residue at position 8 is modified with a cholesterol (Chol) moiety. HIV-1 C-peptide conjugated to cholesterol contain covalent side chain-side chain crosslinks to promote an alpha-helical conformation. The cholesterol-conjugated C-peptides proved to be potent inhibitors of Ebola virus glycoprotein (GP)-mediated cell entry (~10^3-fold reduction in infection at 40 microM
Stereochemistry L
Physicochemical Information
Formula C34H66N12O10S1
Absent amino acids ADEFHILMNOPQRTUVWY
Common amino acids K
Mass 835.03
Pl 10.04
Basic residues 4
Acidic residues 0
Hydrophobic residues 0
Net charge 4
Boman Index 2.8
Hydrophobicity -1.837
Aliphatic Index 0
Half Life
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 6
Literature Information
Literature 1
Title C-peptide inhibitors of Ebola virus glycoprotein-mediated cell entry: effects of conjugation to cholesterol and side chain-side chain crosslinking
Pubmed ID 23962564
Reference Bioorg Med Chem Lett. 2013;23(19):5356-60.
Author Higgins CD, Koellhoffer JF, Chandran K
To the extent possible under law, the person who associated CC0 with this work has waived all copyright and related or neighboring rights to this work.