To the extent possible under law, the person who associated CC0 with this work has waived all copyright and related or neighboring rights to this work.
General Information
DRAVP ID DRAVPe02328
Peptide Name eVpeD1
Sequence YIVPWNGGSRLIRNSRCG
Sequence Length 18
UniProt ID No entry found
Taxon ID None
Source Synthetic construct
Validation Experimentally Validated
Origin Information
Gene Name/ID Not Available
GenBank Not Available
Amino Acid position Not Available
Domain Accession ID Not Available
Nucleotide sequence ID Not Available
Molecular Type Not Available
Chromosomal Position Not Available
Activity Information
Target Organism EBOV
Assay N/A
Activity
Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry
Cytotoxicity No cytotoxicity information found in the reference(s) presented
Binding Target VP24
Mechanism eVpeD1 works against the Ebola virus by inhibiting the protein-protein interaction (PPI) between the viral protein VP24 and the host protein karyopherin alpha 5 (KPNA5). VP24 interferes with the host's immune response by binding to KPNA5, which is crucial for transporting antiviral signaling proteins into the nucleus. By binding to VP24, eVpeD1 prevents its interaction with KPNA5, thereby restoring the normal function of the immune signaling pathway. This inhibition was demonstrated in vitro using an AlphaLISA-based binding assay, where eVpeD1 disrupted the VP24-KPNA5 interaction. Consequently, eVpeD1 and similar peptides have potential as novel antiviral agents against Ebola virus by targeting and blocking this critical PPI.
Structure Information
PDB ID None
Linear/Cyclic Linear
N-terminal Modification Free
C-terminal Modification Acetylation
Other Modification In the peptide sequence, the N-terminus is acetylated, and the tyrosine residue at position 1 is in the D-configuration.)
Stereochemistry L
Physicochemical Information
Formula C89H142N30O24S1
Absent amino acids ADEFHKMOQTU
Common amino acids GR
Mass 2048.35
Pl 10.76
Basic residues 3
Acidic residues 0
Hydrophobic residues 5
Net charge 3
Boman Index 2.2
Hydrophobicity -0.422
Aliphatic Index 81.11
Half Life
Extinction Coefficient cystines 6990
Absorbance 280nm 3.413
Polar residues 9
Literature Information
Literature 1
Title Macrocyclic peptide inhibitors for the protein-protein interaction of Zaire Ebola virus protein 24 and karyopherin alpha 5.
Pubmed ID 28574091
Reference Org Biomol Chem. 2017;15(24):5155-5160.
Author Song X, Lu LY, Passioura T, et al.
To the extent possible under law, the person who associated CC0 with this work has waived all copyright and related or neighboring rights to this work.