DRAVP ID  DRAVPa2329

Peptide Name   Sequence 1 from CN 118767104 A

Sequence  KYGPTPVRDGFK

Sequence Length  12

Source  Synthetic construct

Target Organism  PEDV

Patent Type  Patent Application

Publication Date  2024-10-15

Patent No  CN 118767104 A

Family Info  CN 118767104 A

Patent Title  Application of pea active peptide in inhibition of porcine epidemic diarrhea virus replication and apoptosis

Comment 

Abstract  The invention discloses an application of pea active peptide in inhibition of porcine epidemic diarrhea virus replication and apoptosis, and relates to the technical field of antiviral biologica.According to the application, synthesized pea active peptide is utilized, toxicity of peptides with different concentrations to Vero cells is determined by adopting a CCK-8 test, and the application of the pea active peptide in inhibition of porcine epidemic diarrhea virus replication and apoptosis is achieved. The inhibition effect of the pea active peptide KYGPTPVRDGFK on the porcine epidemic diarrhea virus in Vero cells is determined through indirect immunofluorescence, virus copy number, immunoblotting and half tissue infection amount, and the inhibition effect of the KYGPTPVRDGFK on Vero cell apoptosis induced by the porcine epidemic diarrhea virus is verified through immunoblotting. Results show that the pea bioactive peptide can significantly inhibit the replication of the porcine epidemic diarrhea virus and significantly reduce the Vero cell apoptosis level induced by the porcine epidemic diarrhea virus, thereby providing a new idea and method for the prevention and treatment of the PEDV.