DRAVP ID  DRAVPa2462

Peptide Name   Sequence 2 from Patent CN117886901A

Sequence  FNGAFIAPDRASFLRGKSMGIQSGV

Sequence Length  25

Source  Synthetic construct

Target Organism  AIV

Patent Type  Patent Application

Publication Date  2024-04-16

Patent No  CN117886901A

Family Info  CN 117886901 A

Patent Title  Chicken T cell antigen epitope peptide of H7N9 subtype avian influenza virus HA protein and application of chicken T cell antigen epitope peptide

Comment 

Abstract  The invention belongs to the technical field of biology, and relates to a chicken T cell antigen epitope peptide of H7N9 subtype avian influenza virus (AIV) HA protein and application of the chicken T cell antigen epitope peptide, and the amino acid sequence of the chicken T cell antigen epitope peptide is shown as SEQ ID NO.1-4. Immunoinformatics is combined with an overlapping peptide library method to systematically screen potential T cell epitopes in H7N9 subtype AIV HA protein, candidate polypeptides are synthesized and stimulate lymphocytes, and peptide fragments with remarkable immunodominance response are identified by detecting the level and proliferation level of IFN-gamma secreted by specific T cells of the H7N9 subtype AIV HA protein stimulated by the polypeptides. And the types of the antigen epitopes of the chicken T cells are identified by further utilizing cell sorting and ELISpot test. The T cell antigen epitope peptide has strong immunogenicity, can be applied to H7N9 virus immunodetection and vaccine research and development, and has potential application value in prevention and control of H7N9 viruses.