DRAVPR ID  DRAVPR0153

Name   Spike protein S2',5HB-H2

Sequence  QKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSGGSGGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGSSGGQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSGGSGGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGSSGGQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSSSGGHHHHHH

Sequence Length  250

Source 

UniProt ID P0DTC2 

Gene S2

Target Organism  SARS CoV-2

Activity 

• [Ref:35757908]SARS-CoV-25Hb inhibits SARS-CoV-2 pseudovirus entry with sub-micromolar IC50. Unlike HR2-based peptides that cannot bind spike in the pre-fusion conformation, 5HB features with the capability of binding to pre-fusion spike. Furthermore, 5HB binds viral HR2 at both serological- and endosomal-pH, highlighting its entry-inhibition capacity when SARS-CoV-2 enters via either cell membrane fusion or endosomal route. Finally, we show that 5HB could neutralize S-mediated entry of the predominant SARS-CoV-2 variants and a wide spectrum of sarbecoviruses. These data provide proof-of-concept evidence that 5HB might be developed for the prevention and treatment of SARS-CoV-2 and other emerging sarbecovirus infections.

PDB ID  7Y9N 

Predicted Structure Download  No predicted structure information available for the entry

Modification  No modifications on the sequence.

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Literature 1

Title   An engineered 5-helix bundle derived from SARS-CoV-2 S2 pre-binds sarbecoviral spike at both serological- and endosomal-pH to inhibit virus entry.

Pubmed ID   35757908

Author   Lin, X., Guo, L., Lin, S., Chen, Z., Yang, F., Yang, J., Wang, L., Wen, A., Duan, Y., Zhang, X., Dai, Y., Yin, K., Yuan, X., Yu, C., He, B., Cao, Y., Dong, H., Li, J., Zhao, Q., Lu, G.