DRAVP ID  DRAVPs013

Peptide Name   P21S8Z

Sequence  LDLTYEZLSLQXVVKXLNESY

Sequence Length  21

Original Sequence  DLTYEZLSLQAVVKALNESY

Source  Synthetic construct (From S2 unit of MERS-Cov)

Validation   Experimentally Validated

Target Organism  MERS-CoV

Assay  Inhibition of MERS-CoV S-Protein-Mediated Cell–Cell Fusion Assay

Activity 

• [Ref.29442512]MERS-CoV:inhibition of cell-cell fusion in Huh-7 cells(EC50=0.63 ± 0.05 μM);
• WT MERS-CoV pseudovirus:inhibition of pseudovirus infection in calu-3 cells(EC50=2.80 ± 0.74 μM);
• Q1020H-MERS-CoV pseudovirus:inhibition of pseudovirus infection in calu-3 cells(EC50=4.15± 0.25 μM);
• Q1020R-MERS-CoV pseudovirus:inhibition of pseudovirus infection in calu-3 cells(EC50=2.49±0.18 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.29442512]Calu-3 cell:CC50>100 μM

Binding Target  membrane

Mechanism  The peptide inhibits MERS-CoV infection and its spike (S) protein-mediated cell–cell fusion.

Linear/Cyclic  Cyclic

N-terminal Modification  Acetylation

C-terminal Modification  Carboxyamidation

Special Amino Acid and Stapling Position  ①The 'X' (position: 12 and 16) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②X (12) and X (16) are cross-linked by hydrocarbon stapling. The 'Z' at position 7 indicates R8 ((R)-octenyl alanine)

Structure Description  Not Available

Stereochemistry  L

Literature 1

Title   Discovery of Hydrocarbon-Stapled Short α-Helical Peptides as Promising Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Fusion Inhibitors

Pubmed ID   29442512

Reference   Journal of medicinal chemistry, 61(5), 2018–2026.

Author   Wang, C., Xia, S., Zhang, P., Zhang, T., Wang, W., Tian, Y., Meng, G., Jiang, S., & Liu, K. (2018).

DOI   10.1021/acs.jmedchem.7b01732