General Information


DRAVP ID  DRAVPe00087

Peptide Name   Vpr 65-79

Sequence  QQLLFIHFRIGCQHS

Sequence Length  15

UniProt ID  No entry found

Source  Synthetic construct(derived from HIV-1 viral protein R (Vpr))



Activity Information


Target Organism  HIV

Assay  Dot-blot binding assay

Activity 

  • [Ref.17490682]HIV-1:inhibit the activity of RNA-dependent DNA polymerase(IC50=33 µM);inhibit the activity of DNA-dependent DNA polymerase(IC50=43 µM);inhibit the activity of Rnase H(IC50=16.5 µM);
  • Inhibition of 3′-end processing catalyzed by integrase(IC50=76 µM);inhibition of strand transfer catalyzed by integrase(IC50=14 µM);inhibition of disintegration catalyzed by integrase(IC50=10 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target  Integrase,Reverse Transcriptase

Mechanism  The viral enzymes RT and IN have cardinal roles in the early stages of the replication of retroviruses and retrotransposons. The peptide derived from viral protein R (Vpr) could inhibit integrase(IN) and Reverse Transcriptase(RT) and thus inhibit the replication of HIV-1.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00087

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C83H127N25O20S

Absent amino acids  ADEKMNPTVWY

Common amino acids  Q

Mass  1827.14

Pl  8.27

Basic residues  3

Acidic residues  0

Hydrophobic residues  6

Net charge  3

Boman Index  -1640

Hydrophobicity  14

Aliphatic Index  104

Half Life 

  •     Mammalian:0.8 hour
  •     Yeast:10 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  3



Literature Information


Literature 1

Title   Inhibition of the activities of reverse transcriptase and integrase of human immunodeficiency virus type-1 by peptides derived from the homologous viral protein R (Vpr).

Pubmed ID   17490682

Reference   J Mol Biol. 2007 Jun 22;369(5):1230-43.

Author   Gleenberg IO, Herschhorn A, Hizi A.

DOI   10.1016/j.jmb.2007.03.073