General Information

General Information

DRAVP ID DRAVPe00108

Peptide Name LCE40

Sequence YQLLIRMI

Sequence Length 8

UniProt ID No entry found

Source Synthetic construct

Activity Information

Target Organism HIV

Assay Disintegration assay

Activity

[Ref.12054767]HIV-1:Inhibition of 3′-end processing catalyzed by integrase(IC50=120 µM);no inhibition of HIV-1 infection up to 100 μM.

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.12054767]P4 cells: Cell viability estimated by the MTT assay was decreased by about 5% at peptide concentrations higher than 100 μM.

Binding Target Integrase

Mechanism Binding of both viral DNA and host chromosomal DNA are critical steps in IN-catalyzed reactions. The peptide interacted with the catalytic domain of IN interfering with the binding of the DNA substrate and inhibit the replication of virus.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00108

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C49H84N12O11S

Absent amino acids ACDEFGHKNPSTVW

Common amino acids IL

Mass 1049.34

Pl 8.75

Basic residues 1

Acidic residues 0

Hydrophobic residues 4

Net charge 1

Boman Index 143

Hydrophobicity 115

Aliphatic Index 195

Half Life

Mammalian:2.8 hour
Yeast:10 min
E.coli:2 min

Extinction Coefficient cystines 1490

Absorbance 280nm 212.86

Polar residues 1

Literature Information

Literature 1

Title A novel short peptide is a specific inhibitor of the human immunodeficiency virus type 1 integrase.

Pubmed ID 12054767

Reference J Mol Biol. 2002 Apr 19;318(1):45-58.

Author de Soultrait VR, Caumont A, Parissi V, Morellet N, Ventura M, Lenoir C, Litvak S, Fournier M, Roques B.

DOI 10.1016/S0022-2836(02)00033-5