General Information

General Information

DRAVP ID DRAVPe00121

Peptide Name P47(derived from E2 envelope protein of GB virus C)

Sequence VELSEWGVPCATCILDRR

Sequence Length 18

UniProt ID No entry found

Source Synthetic construct(derived from E2 envelope protein of GB virus C)

Activity Information

Target Organism HIV

Assay Gp41-Mediated Cell-Cell Fusion Assay

Activity

[Ref.20718496]HIV-1:inhibition of gp41-induced cell-cell fusion in CEM-174 cells(IC50=330.8 μM);inhibition of HIV(HXB2) infection in TZM-bl Cells(IC50=58.6 μM);inhibition of HIV(BAL) infection in TZM-bl Cells(IC50=140.3 μM);inhibition of HIV(69-7) infection in TZM-bl Cells(IC50=20.1 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target membrane

Mechanism E2 GBV-C domain interferes with the HIV-1 fusion peptide-vesicle interaction, produce a notable decrease the cellular membrane fusion, and interfere with the HIV-1 infectivity in a dose-dependent manner.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00121

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C88H143N25O27S2

Absent amino acids FHKMNQY

Common amino acids CELRV

Mass 2047.37

Pl 4.68

Basic residues 2

Acidic residues 3

Hydrophobic residues 7

Net charge -1

Boman Index -2767

Hydrophobicity 18.89

Aliphatic Index 102.78

Half Life

Mammalian:100 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5625

Absorbance 280nm 330.88

Polar residues 5

Literature Information

Literature 1

Title Effect of synthetic peptides belonging to E2 envelope protein of GB virus C on human immunodeficiency virus type 1 infection.

Pubmed ID 20718496

Reference J Med Chem. 2010 Aug 26;53(16):6054-63.

Author Herrera E, Tenckhoff S, Gómara MJ, Galatola R, Bleda MJ, Gil C, Ercilla G, Gatell JM, Tillmann HL, Haro I.

DOI 10.1021/jm100452c