General Information

General Information

DRAVP ID DRAVPe00127

Peptide Name VMI7

Sequence SNQGGSPLPRSV

Sequence Length 12

UniProt ID No entry found

Source Synthetic construct(phage display)

Activity Information

Target Organism HIV

Assay Affinity binding assay

Activity

[Ref.12480936]HIV-1:inhibition of Vif-Vif bingding(IC50=7.43 μM);
The peptide is able to effectively inhibit HIV-1 replication At the concentration of 50 μM.

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.12480936]No significant cytotoxicity on H9 cells up to 50 μM.

Binding Target Vif proteins

Mechanism Vif proteins are essential for HIV-1 replication and able to form multimer, which is critical to the biological activity of many prokaryotic and eukaryotic proteins and is a common mechanism for the functional activation/inactivation of proteins.The peptide could bind with vif proteins and block the multimerization of them,which should inhibit HIV-1 replication.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00127

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C49H83N17O18

Absent amino acids ACDEFHIKMTWY

Common amino acids S

Mass 1198.3

Pl 9.47

Basic residues 1

Acidic residues 0

Hydrophobic residues 2

Net charge 1

Boman Index -2646

Hydrophobicity -82.5

Aliphatic Index 56.67

Half Life

Mammalian:1.9 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 6

Literature Information

Literature 1

Title Potent suppression of viral infectivity by the peptides that inhibit multimerization of human immunodeficiency virus type 1 (HIV-1) Vif proteins.

Pubmed ID 12480936

Reference J Biol Chem. 2003 Feb 21;278(8):6596-602.

Author Yang B, Gao L, Li L, Lu Z, Fan X, Patel CA, Pomerantz RJ, DuBois GC, Zhang H.

DOI 10.1074/jbc.M210164200