General Information


DRAVP ID  DRAVPe00222

Peptide Name   VIRIP[A13,C6,C11,F12]

Sequence  LEAIPCSIPPCFAFNKPFVF

Sequence Length  20

UniProt ID  No entry found

Source  Synthetic construct(derived from α1-antitrypsin)



Activity Information


Target Organism  HIV

Assay  ELISA

Activity 

  • [Ref.17448989]HIV-1:inhibition of virus infection in P4-CCR5 clls(IC50=0.27±0.04 µM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.17448989]No cytotoxicity against P4-CCR5 cells up tp 1000 µM.

Binding Target  membrane

Mechanism  The peptide can inhibit HIV-1 entry by binding to the gp41 FP and preventing its insertion into the target cell membrane.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00222

Linear/Cyclic  Cyclic

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Disulfide bond between Cys6 and Cys11

Stereochemistry  L



Physicochemical Information


Formula  C109H158N22O25S2

Absent amino acids  DGHMQRTWY

Common amino acids  FP

Mass  2240.71

Pl  5.99

Basic residues  1

Acidic residues  1

Hydrophobic residues  10

Net charge  0

Boman Index  1450

Hydrophobicity  93.5

Aliphatic Index  83

Half Life 

  •     Mammalian:5.5 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  125

Absorbance 280nm  6.58

Polar residues  4



Literature Information


Literature 1

Title   Discovery and optimization of a natural HIV-1 entry inhibitor targeting the gp41 fusion peptide.

Pubmed ID   17448989

Reference   Cell. 2007 Apr 20;129(2):263-75.

Author   Münch J, Ständker L, Adermann K, Schulz A, Schindler M, Chinnadurai R, Pöhlmann S, Chaipan C, Biet T, Peters T, Meyer B, Wilhelm D, Lu H, Jing W, Jiang S, Forssmann WG, Kirchhoff F.

DOI   10.1016/j.cell.2007.02.042