General Information

General Information

DRAVP ID DRAVPe00255

Peptide Name 651

Sequence MTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELL

Sequence Length 36

UniProt ID No entry found

Source Synthetic construct

Other Link DRAVPa0971

Activity Information

Target Organism HIV

Assay MAGI/cMAGI infectivity assay

Activity

[Ref.17640899]HIV IIIB:inhibition of virus infection on CEM4 cells(IC50=0.008 μg/ml);
HIV 098:inhibition of virus infection on CEM4 cells(IC50=0.033μg/ml);
HIV 098-T20:inhibition of virus infection on CEM4 cells(IC50=0.060 μg/ml);
HIV 098-T1249:inhibition of virus infection on CEM4 cells(IC50=0.151 μg/ml);
HIV 098-T651:inhibition of virus infection on CEM4 cells(IC50=7.599 μg/ml).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

No cytotoxicity information or data found in the reference(s) presented in this entry

Binding Target membrane

Mechanism The peptide acts by binding to the heptad repeat 1 (HR1) region of gp41 and preventing the interaction of the HR1 and HR2 domains, which is required for virus–cell fusion.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00255

Linear/Cyclic Linear

N-terminal Modification Acetylation

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C193H296N52O67S2

Absent amino acids ACFGPV

Common amino acids E

Mass 4480.9

Pl 4.21

Basic residues 3

Acidic residues 8

Hydrophobic residues 9

Net charge -5

Boman Index -10192

Hydrophobicity -116.67

Aliphatic Index 75.83

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 12490

Absorbance 280nm 356.86

Polar residues 10

Literature Information

Literature 1

Title Design of helical, oligomeric HIV-1 fusion inhibitor peptides with potent activity against enfuvirtide-resistant virus.

Pubmed ID 17640899

Reference Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12772-7.

Author Dwyer JJ, Wilson KL, Davison DK, Freel SA, Seedorff JE, Wring SA, Tvermoes NA, Matthews TJ, Greenberg ML, Delmedico MK.

DOI 10.1073/pnas.0701478104