General Information

General Information

DRAVP ID DRAVPe00296

Peptide Name Kalata-B1 (Plant defensin)

Sequence GLPVCGETCVGGTCNTPGCTCSWPVCTRN

Sequence Length 29

UniProt ID P56254

Source Oldenlandia affinis

Activity Information

Target Organism HIV

Assay

Activity

[Ref.18008336]HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=140 nM).

Hemolytic Activity

[Ref.12779323] HD50 = 300 μM against Human type A red blood cells.

Cytotoxicity

[Ref.18008336]CEM-SS cells:IC50=3500 nM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID 2KHB 1NB1

Predicted Structure Download DRAVPe00296

Linear/Cyclic Cyclic

N-terminal Modification Cyclization (N termini to C termini)

C-terminal Modification Cyclization (C termini to N termini)

Other Modification Disulfide bonds between Cys5 and Cys19, Cys9 and Cys21, Cys14 and Cys26.

Stereochemistry L

Physicochemical Information

Formula C117H187N35O40S6

Absent amino acids ADFHIKMQY

Common amino acids C

Mass 2916.34

Pl 5.96

Basic residues 1

Acidic residues 1

Hydrophobic residues 5

Net charge 0

Boman Index -1951

Hydrophobicity 15.17

Aliphatic Index 43.45

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5875

Absorbance 280nm 209.82

Polar residues 19

Literature Information

Literature 1

Title Cyclotides as natural anti-HIV agents.

Pubmed ID 18008336

Reference Biopolymers. 2008;90(1):51-60.

Author Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.

DOI 10.1002/bip.20886

Literature 2

Title Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity.

Pubmed ID 12779323

Reference Biochemistry. 2003 Jun 10;42(22):6688-95.

Author Barry DG, Daly NL, Clark RJ, Sando L, Craik DJ.

DOI 10.1021/bi027323n