General Information

General Information

DRAVP ID DRAVPe00339

Peptide Name Brevinin-2-related peptide (Frogs, amphibians, animals)

Sequence GIWDTIKSMGKVFAGKILQNL

Sequence Length 21

UniProt ID No entry found

Source Rana septentrionalis (mink frog)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.20086159]HIV-1 IIIB:inhibition the cytopathic effects of HIV-1 infection in CEM-SS cells(EC50=1.65 μM).

Hemolytic Activity

[Ref.15556063]HC50=70 µM against human erythrocytes

Cytotoxicity

[Ref.20086159]CEM-SS cells:50% Cell death at 7.42 µM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00339

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C107H175N27O28S

Absent amino acids CEHPRY

Common amino acids GIK

Mass 2319.79

Pl 9.7

Basic residues 3

Acidic residues 1

Hydrophobic residues 9

Net charge 2

Boman Index -259

Hydrophobicity 28.57

Aliphatic Index 111.43

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 5500

Absorbance 280nm 275

Polar residues 6

Literature Information

Literature 1

Title Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database.

Pubmed ID 20086159

Reference Antimicrob Agents Chemother. 2010 Mar;54(3):1343-6.

Author Wang G, Watson KM, Peterkofsky A, Buckheit RW Jr.

DOI 10.1128/AAC.01448-09

Literature 2

Title Purification and characterization of antimicrobial peptides from the skin secretions of the mink frog (Rana septentrionalis).

Pubmed ID 15556063

Reference Comp Biochem Physiol C Toxicol Pharmacol. 2004 Oct;139(1-3):31-38.

Author Bevier CR, Sonnevend A, Kolodziejek J, Nowotny N, Nielsen PF, Conlon JM.

DOI 10.1016/j.cca.2004.08.019