General Information

General Information

DRAVP ID DRAVPe00343

Peptide Name Ascaphin-8 (Frogs, amphibians, animals)

Sequence GFKDLLKGAAKALVKTVLF

Sequence Length 19

UniProt ID P0CJ32

Source Ascaphus truei (Coastal tailed frog)

Activity Information

Target Organism HIV

Assay

Activity

[Ref.20086159]HIV-1 IIIB:inhibition the cytopathic effects of HIV-1 infection in CEM-SS cells(EC50=1.2 μM).

Hemolytic Activity

[Ref.15207717]HC50=50 μM against human erythrocytes

Cytotoxicity

[Ref.20086159]CEM-SS cells:50% Cell death at 2.9 µM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00343

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C97H163N23O23

Absent amino acids CEHIMNPQRSWY

Common amino acids KL

Mass 2019.5

Pl 10

Basic residues 4

Acidic residues 1

Hydrophobic residues 11

Net charge 3

Boman Index 754

Hydrophobicity 73.68

Aliphatic Index 128.42

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 3

Literature Information

Literature 1

Title Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database.

Pubmed ID 20086159

Reference Antimicrob Agents Chemother. 2010 Mar;54(3):1343-6.

Author Wang G, Watson KM, Peterkofsky A, Buckheit RW Jr.

DOI 10.1128/AAC.01448-09

Literature 2

Title The ascaphins: a family of antimicrobial peptides from the skin secretions of the most primitive extant frog, Ascaphus truei.

Pubmed ID 15207717

Reference Biochem Biophys Res Commun. 2004 Jul 16;320(1):170-175.

Author Conlon JM, Sonnevend A, Davidson C, Smith DD, Nielsen PF.

DOI 10.1016/j.bbrc.2004.05.141