General Information

General Information

DRAVP ID DRAVPe00354

Peptide Name Caerin 1.9

Sequence GLFGVLGSIAKHVLPHVVPVIAEKL

Sequence Length 25

UniProt ID P81252

Source Litoria chloris

Activity Information

Target Organism HIV

Assay

Activity

[Ref.26026377]Human immunodeficiency virus (HIV): inhibition of HIV Pseudovirus (PsV) infection in CD4+ T cells(IC50=4 µM).
[Ref.16140737]Human immunodeficiency virus (HIV):inhibit 50% of PBS-treated HIV infection of T cells(IC50=1.2 μM);inhibition of HIV transfer by dendritic cells to T cells(IC50=1.6 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.26026377]CD4+ T Lymphocytes:50% cell death at 20 µM.

Binding Target virion envelope

Mechanism Prevent HIV infection by disrupting the integrity of the virion membrane at concentrations that are not toxic to target cells, the peptide also inhibit the transfer of HIV from DCs to T cells.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00354

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C124H205N31O29

Absent amino acids CDMNQRTWY

Common amino acids V

Mass 2594.18

Pl 8.61

Basic residues 4

Acidic residues 1

Hydrophobic residues 14

Net charge 3

Boman Index 2851

Hydrophobicity 114.8

Aliphatic Index 159.6

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 4

Literature Information

Literature 1

Title VanCompernolle S, Smith PB, Bowie JH, Tyler MJ, Unutmaz D, Rollins-Smith LA.

Pubmed ID 26026377

Reference Peptides. 2015 Sep;71:296-303.

Author Inhibition of HIV infection by caerin 1 antimicrobial peptides.

DOI 10.1016/j.peptides.2015.05.004

Literature 2

Title VanCompernolle SE, Taylor RJ, Oswald-Richter K, Jiang J, Youree BE, Bowie JH, Tyler MJ, Conlon JM, Wade D, Aiken C, Dermody TS, KewalRamani VN, Rollins-Smith LA, Unutmaz D.

Pubmed ID 16140737

Reference J Virol. 2005 Sep;79(18):11598-606.

Author Antimicrobial peptides from amphibian skin potently inhibit human immunodeficiency virus infection and transfer of virus from dendritic cells to T cells.

DOI 10.1128/JVI.79.18.11598-11606.2005

Literature 3

Title Rollins-Smith LA, Smith PB, Ledeczi AM, Rowe JM, Reinert LK.

Pubmed ID 33008028

Reference Antibiotics (Basel). 2020 Sep 30;9(10):661.

Author Caerin 1 Antimicrobial Peptides That Inhibit HIV and Neisseria May Spare Protective Lactobacilli.

DOI 10.3390/antibiotics9100661