General Information

General Information

DRAVP ID DRAVPe00370

Peptide Name PGLa (frog)

Sequence GMASKAGAIAGKIAKVALKAL

Sequence Length 21

UniProt ID Q99134

Taxon ID None

Source Xenopus laevis

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism HIV,CCV

Assay

Activity

[Ref.20086159]HIV-1 IIIB:inhibition the cytopathic effects of HIV-1 infection in CEM-SS cells(EC50>50.8 μM).
[Ref.15193922]Channel Catfish virus(CCV): inhibition of CCV infection in catfish ovary(CCO) cells(reduce viral infectivity by 50% at 100 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.20086159]CEM-SS cells:TC50(cause 50% Cell death) >50.8 µM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00370

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C88H161N25O23S

Absent amino acids CDEFHNPQRTWY

Common amino acids A

Mass 1969.46

Pl 10.48

Basic residues 4

Acidic residues 0

Hydrophobic residues 12

Net charge 4

Boman Index 1596

Hydrophobicity 84.29

Aliphatic Index 121.43

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 4

Literature Information

Literature 1

Title Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database.

Pubmed ID 20086159

Reference Antimicrob Agents Chemother. 2010 Mar;54(3):1343-6.

Author Wang G, Watson KM, Peterkofsky A, Buckheit RW Jr.

DOI 10.1128/AAC.01448-09

Literature 2

Title Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides.

Pubmed ID 15193922

Reference Virology. 2004 Jun 1;323(2):268-75.

Author Chinchar VG, Bryan L, Silphadaung U, Noga E, Wade D, Rollins-Smith L.

DOI 10.1016/j.virol.2004.02.029