General Information

General Information

DRAVP ID DRAVPe00370

Peptide Name PGLa (frog)

Sequence GMASKAGAIAGKIAKVALKAL

Sequence Length 21

UniProt ID Q99134

Taxon ID

Source Xenopus laevis

Validation

Origin Information

Gene Name/ID

GenBank

Amino Acid position

Domain Accession ID

Nucleotide sequence ID

Molecular Type

Chromosomal Position

Activity Information

Target Organism HIV,CCV

Assay

Activity

[Ref.20086159]HIV-1 IIIB:inhibition the cytopathic effects of HIV-1 infection in CEM-SS cells(EC50>50.8 μM).
[Ref.15193922]Channel Catfish virus(CCV): inhibition of CCV infection in catfish ovary(CCO) cells(reduce viral infectivity by 50% at 100 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.20086159]CEM-SS cells:TC50(cause 50% Cell death) >50.8 µM.

Binding Target Not found

Mechanism No machanism information found in the reference(s) presented in this entry

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00370

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C88H161N25O23S

Absent amino acids CDEFHNPQRTWY

Common amino acids A

Mass 1969.46

Pl 10.48

Basic residues 4

Acidic residues 0

Hydrophobic residues 12

Net charge 4

Boman Index 1596

Hydrophobicity 84.29

Aliphatic Index 121.43

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 4

Literature Information

Literature 1

Title Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database.

Pubmed ID 20086159

Reference Antimicrob Agents Chemother. 2010 Mar;54(3):1343-6.

Author Wang G, Watson KM, Peterkofsky A, Buckheit RW Jr.

DOI 10.1128/AAC.01448-09

Literature 2

Title Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides.

Pubmed ID 15193922

Reference Virology. 2004 Jun 1;323(2):268-75.

Author Chinchar VG, Bryan L, Silphadaung U, Noga E, Wade D, Rollins-Smith L.

DOI 10.1016/j.virol.2004.02.029