General Information


DRAVP ID  DRAVPe00421

Peptide Name   EK1V2

Sequence  SLDQINVTFLDLEYEMKKLEEAIKKLEESYIDLKEL

Sequence Length  36

UniProt ID  No entry found

Source  Synthetic construct(derived from EK1)



Activity Information


Target Organism  SARS-CoV-2,SARS-CoV,MERS-CoV,HCoV-NL63

Assay 

Activity 

  • [Ref.34344868]SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=0.9±0.2 nM);inhibition of SARS-CoV-2 pseudovirus infection in Huh-7 cells(IC50=87.2±8.3 nM);
  • SARS-CoV-2 D614G:inhibition of S protein-mediated cell-cell fusion(IC50=0.5±0.2 nM);inhibition of pseudovirus infection in Huh-7 cells(IC50=106.5±5.4 nM);
  • SARS-CoV:inhibition of pseudovirus infection in Huh-7 cells(IC50=252±5.6 nM);
  • MERS-CoV:inhibition of pseudovirus infection in Huh-7 cells(IC50=1.1±0.3 nM);
  • HCoV-NL63:inhibition of pseudovirus infection in Huh-7 cells(IC50=59.4±13.1 nM);
  • HCoV-229E:inhibition of pseudovirus infection in Huh-7 cells(IC50=503.3±20.9 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  membrane

Mechanism  A six-helical bundle structure is formed by two heptad repeat domains (HR1 and HR2) in S2, juxtaposing the viral and cellular membranes for fusion.The peptide derived from the HR2 sequence can competitively bind to the viral HR1 domain thus exerting antiviral activity.



Structure Information


PDB ID  None

Predicted Structure Download  No predicted structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  PEG8-K(Chol)

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C196H317N43O64S

Absent amino acids  CGHPRW

Common amino acids  EL

Mass  4331.98

Pl  4.36

Basic residues  5

Acidic residues  10

Hydrophobic residues  13

Net charge  -5

Boman Index  -6303

Hydrophobicity  -43.33

Aliphatic Index  119.17

Half Life 

  •     Mammalian:1.9 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  2980

Absorbance 280nm  85.14

Polar residues  6



Literature Information


Literature 1

Title   SARS-CoV-2-derived fusion inhibitor lipopeptides exhibit highly potent and broad-spectrum activity against divergent human coronaviruses.

Pubmed ID   34344868

Reference   Signal Transduct Target Ther. 2021 Aug 3;6(1):294.

Author   Zhu Y, Yu D, Hu Y, Wu T, Chong H, He Y.

DOI   10.1038/s41392-021-00698-x