General Information

General Information

DRAVP ID DRAVPe00453

Peptide Name M2 MH

Sequence RKFFKKIYRFFRKLLKRL

Sequence Length 18

UniProt ID No entry found

Taxon ID None

Source Synthetic construct(derived from influenza virus M2 protein)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism Influenza virus

Assay

Activity

[Ref.31375212]H1N1(A/Puerto Rico/8/34):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=53 nM);
H3N2(A/Sydney/5/97):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.058 μM);
H3N2(A/X31):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.016 μM);
H5N2(A/aquatic bird/Korea/w81/2005 ):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.066 μM);
H1N1(A/Swine/Iowa/15/30):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.053 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.31375212]Madin-Darby canine kidney epithelial(MDCK) cells:CC50=19.2 μM

Binding Target membrane

Mechanism M2 MH is an α-helical amphipathic peptide with basic amino acids positioned at the polar-nonpolar interface. The peptide inserts deeply into the hydrophobic core of the membrane bilayer, resulting in alteration of membrane order and curvature . This ability to alter membrane curvature enables M2 MH to induce membrane budding in vitro and in vivo. It localizes to the neck of the budding virion and plays an important role in membrane scission.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00453

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C123H199N35O20

Absent amino acids ACDEGHMNPQSTVW

Common amino acids K

Mass 2488.16

Pl 12.02

Basic residues 9

Acidic residues 0

Hydrophobic residues 8

Net charge 9

Boman Index -5597

Hydrophobicity -65

Aliphatic Index 86.67

Half Life

Mammalian:1 hour
Yeast:2 min
E.coli:2 min

Extinction Coefficient cystines 1490

Absorbance 280nm 87.65

Polar residues 1

Literature Information

Literature 1

Title Envelope-deforming antiviral peptide derived from influenza virus M2 protein.

Pubmed ID 31375212

Reference Biochem Biophys Res Commun. 2019 Sep 24;517(3):507-512.

Author Jung Y, Kong B, Moon S, Yu SH, Chung J, Ban C, Chung WJ, Kim SG, Kweon DH.

DOI 10.1016/j.bbrc.2019.07.088