DRAVP ID  DRAVPe00453

Peptide Name   M2 MH

Sequence  RKFFKKIYRFFRKLLKRL

Sequence Length  18

UniProt ID  No entry found

Source  Synthetic construct(derived from influenza virus M2 protein)

Target Organism  Influenza virus

Assay 

Activity 

• [Ref.31375212]H1N1(A/Puerto Rico/8/34):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=53 nM);
• H3N2(A/Sydney/5/97):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.058 μM);
• H3N2(A/X31):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.016 μM);
• H5N2(A/aquatic bird/Korea/w81/2005 ):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.066 μM);
• H1N1(A/Swine/Iowa/15/30):inhibition of infection in Madin-Darby canine kidney epithelial(MDCK) cells(IC50=0.053 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.31375212]Madin-Darby canine kidney epithelial(MDCK) cells:CC50=19.2 μM

Binding Target  membrane

Mechanism  M2 MH is an α-helical amphipathic peptide with basic amino acids positioned at the polar-nonpolar interface. The peptide inserts deeply into the hydrophobic core of the membrane bilayer, resulting in alteration of membrane order and curvature . This ability to alter membrane curvature enables M2 MH to induce membrane budding in vitro and in vivo. It localizes to the neck of the budding virion and plays an important role in membrane scission.

PDB ID  None

Predicted Structure Download  DRAVPe00453

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L

Literature 1

Title   Envelope-deforming antiviral peptide derived from influenza virus M2 protein.

Pubmed ID   31375212

Reference   Biochem Biophys Res Commun. 2019 Sep 24;517(3):507-512.

Author   Jung Y, Kong B, Moon S, Yu SH, Chung J, Ban C, Chung WJ, Kim SG, Kweon DH. 

DOI   10.1016/j.bbrc.2019.07.088