General Information

General Information

DRAVP ID DRAVPe00456

Peptide Name Hs-1

Sequence FLPLILPSIVTALSSFLKQG

Sequence Length 20

UniProt ID No entry found

Source Hypsiboas semilineatus

Activity Information

Target Organism DENV

Assay

Activity

[Ref.29153860]DENV2:reduced the number of lysis plaques by 100% at 125 µg/mL; by 60% at 7.81 μg/mL;
DENV3:reduced the number of lysis plaques by 100% at 125 µg/mL; by 50% at 7.81 μg/mL

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.29153860]No significant cytotoxicity agaisnt Vero cells up to 125μg/ mL.

Binding Target envelope

Mechanism It was possible to observe that the viral particle lost its ability to infect because of the breakdown on the envelope as the peptide acted directly on the virus.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00456

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C104H170N22O26

Absent amino acids CDEHMNRWY

Common amino acids L

Mass 2144.63

Pl 8.75

Basic residues 1

Acidic residues 0

Hydrophobic residues 11

Net charge 1

Boman Index 2333

Hydrophobicity 127.5

Aliphatic Index 156

Half Life

Mammalian:1.1 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 5

Literature Information

Literature 1

Title The antimicrobial peptide HS-1 inhibits dengue virus infection.

Pubmed ID 29153860

Reference Virology. 2018 Jan 15;514:79-87.

Author Monteiro JMC, Oliveira MD, Dias RS, Nacif-Marçal L, Feio RN, Ferreira SO, Oliveira LL, Silva CC, Paula SO.

DOI 10.1016/j.virol.2017.11.009