General Information

General Information

DRAVP ID DRAVPe00467

Peptide Name WN53

Sequence TFLVHREWFMDLNLPWSSAGSTVWR

Sequence Length 25

UniProt ID No entry found

Source Synthetic construct(derived from the E polyprotein of WNV)

Activity Information

Target Organism WNV

Assay

Activity

[Ref.31351847]West Nile virus(WNV): inhibition of WNV infection in LLCKM-2 monkey kidney epithelial cells(IC50~10μM,56.0±3.0% inhibition at 99μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.31351847]No cytotoxicity against LLCMK-2 monkey kidney epithelial cells at 100 mg/ml.

Binding Target envelope

Mechanism The peptide may play roles in protein-protein rearrangements or bilayer membrane interactions during the entry and fusion process and thus inhibit infection of DENV or WNV.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00467

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C142H203N37O36S

Absent amino acids CIKQY

Common amino acids LSW

Mass 3036.46

Pl 6.42

Basic residues 3

Acidic residues 2

Hydrophobic residues 11

Net charge 1

Boman Index -3112

Hydrophobicity -8.4

Aliphatic Index 74

Half Life

Mammalian:7.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 16500

Absorbance 280nm 687.5

Polar residues 7

Literature Information

Literature 1

Title Peptide derivatives as inhibitors of NS2B-NS3 protease from Dengue, West Nile, and Zika flaviviruses.

Pubmed ID 31351847

Reference Bioorg Med Chem. 2019 Sep 15;27(18):3963-3978.

Author da Silva-Júnior EF, de Araújo-Júnior JX.

DOI 10.1016/j.bmc.2019.07.038