General Information

General Information

DRAVP ID DRAVPe00468

Peptide Name WN83

Sequence TFLVHREWFMDLNLPWSSA

Sequence Length 19

UniProt ID No entry found

Source Synthetic construct(derived from the E polyprotein of WNV)

Activity Information

Target Organism WNV

Assay

Activity

[Ref.31351847]West Nile virus(WNV): inhibition of WNV infection in LLCKM-2 monkey kidney epithelial cells(IC50~10μM, 70.0±3.0% inhibition at 128 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.31351847]No cytotoxicity against LLCMK-2 monkey kidney epithelial cells at 100 mg/ml.

Binding Target envelope

Mechanism The peptide may play roles in protein-protein rearrangements or bilayer membrane interactions during the entry and fusion process and thus inhibit infection of DENV or WNV.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00468

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C111H157N27O28S

Absent amino acids CGIKQY

Common amino acids L

Mass 2349.69

Pl 5.29

Basic residues 2

Acidic residues 2

Hydrophobic residues 9

Net charge 0

Boman Index -1754

Hydrophobicity 5.26

Aliphatic Index 82.11

Half Life

Mammalian:7.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 11000

Absorbance 280nm 611.11

Polar residues 4

Literature Information

Literature 1

Title Peptide derivatives as inhibitors of NS2B-NS3 protease from Dengue, West Nile, and Zika flaviviruses.

Pubmed ID 31351847

Reference Bioorg Med Chem. 2019 Sep 15;27(18):3963-3978.

Author da Silva-Júnior EF, de Araújo-Júnior JX.

DOI 10.1016/j.bmc.2019.07.038