DRAVP ID  DRAVPe00469

Peptide Name   AR-23

Sequence  AIGSILGALAKGLPTLISWIKNR

Sequence Length  23

UniProt ID  A7BJK6 

Source  Rana tagoi (Tago frog)

Target Organism  HSV,MeV,HPIV,HCoV-229E

Assay 

Activity 

• [Ref.35055066]HSV-1: Co-treatment:simultaneous addition of AR-23 and virus to the cells(IC50=0.78 μM);Virus pre-treatment: virus incubated with the peptide(IC50=0.39 μM);Cell pre-treatment: AR-23 incubated with the cells before the viral infection and Post-treatment: AR-23 added to the infected cells(AR-23 had a slight inhibitory effect);inhibition the entry of virus(IC50=3.125μM);inhibition of viral attachment on the host cell membrane(IC50=6.25 μM);
• Measles virus (MeV):Co-treatment: simultaneous addition of AR-23 and virus to the cells(IC50=6.25 μM);Virus pre-treatment: virus incubated with the peptide(IC50=3.125 μM);
• Human parainfluenza virus type-2 (HPIV2):Co-treatment: simultaneous addition of AR-23 and virus to the cells(IC50=6.25 μM);Virus pre-treatment: virus incubated with the peptide(IC50=3.125 μM);
• HCoV-229E:Virus pre-treatment: virus incubated with the peptide(IC50=3.125 μM);
• SARS-CoV-2:Co-treatment:simultaneous addition of AR-23 and virus to the cells(60% of inhibition at 25 μM);Virus pre-treatment: virus incubated with the peptide(IC50=12.5 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.35055066]Vero cells:CC50=25 μM; >80% cytotoxic against Vero cells at 100 and 50 µM.

Binding Target  envelope glycoprotein

Mechanism  No machanism information found in the reference(s) presented in this entry

PDB ID  6dst 

Predicted Structure Download  DRAVPe00469

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L

Literature 1

Title   The Broad-Spectrum Antiviral Potential of the Amphibian Peptide AR-23.

Pubmed ID   35055066

Reference   Int J Mol Sci. 2022 Jan 14;23(2):883.

Author   Chianese A, Zannella C, Monti A, De Filippis A, Doti N, Franci G, Galdiero M.

DOI   10.3390/ijms23020883