General Information

General Information

DRAVP ID DRAVPe00474

Peptide Name ACE2 (27-42)(SAP1)

Sequence TFLDKFNHEAEDLFYQ

Sequence Length 16

UniProt ID Q9BYF1

Source Synthetic construct

Activity Information

Target Organism SARS-CoV-2,SARS-CoV,HCoV-OC43,VSV

Assay pseudovirus inhibition assay

Activity

[Ref.33356169]SARS-CoV-2:Inhibition of infection in 293T/ACE2 cells(IC50=2.39±0.20 mM);Inhibition of infection in 293T/ACE2/GFP cells(IC50=3 mM);
SARS-CoV:Inhibition of infection in 293T/ACE2 cells(80% inhibition at 3 mM);
Vesicular Stomatitis Virus (VSV):No nhibition of infection in 293T/ACE2 cells up to 3 mM;
HCoV-NL63:Inhibition of cytopathic effect in LLC-MK2 cells(30% Inhibition at 3 mM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target Not found

Mechanism Inhibiting SARS-CoV-2 infection by disrupting the Spike-ACE2 interaction interface with peptide-based inhibitors.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe00474

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C94H129N21O29

Absent amino acids CGIMPRSVW

Common amino acids F

Mass 2017.18

Pl 4.31

Basic residues 2

Acidic residues 4

Hydrophobic residues 6

Net charge -2

Boman Index -3557

Hydrophobicity -76.88

Aliphatic Index 55

Half Life

Mammalian:7.2 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 1490

Absorbance 280nm 99.33

Polar residues 3

Literature Information

Literature 1

Title Rationally Designed ACE2-Derived Peptides Inhibit SARS-CoV-2.

Pubmed ID 33356169

Reference Bioconjug Chem. 2021 Jan 20;32(1):215-223.

Author Larue RC, Xing E, Kenney AD, Zhang Y, Tuazon JA, Li J, Yount JS, Li PK, Sharma A.

DOI 10.1021/acs.bioconjchem.0c00664