General Information


DRAVP ID  DRAVPe00518

Peptide Name   M1

Sequence  EANTTLLDLTYEMLSLQQVVKALNESYIDLKEL

Sequence Length  33

UniProt ID  No entry found

Source  Synthetic construct



Activity Information


Target Organism  MERS-CoV

Assay  ELISA

Activity 

  • [Ref.27795416]MERS-CoV:inhibition of MERS-CoV S-medicated cell-cell fusion in MT-2 cells(IC50=0.9 μM);inhibition of pseudovirus infection in MT-2 cells(IC50=2.3 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  membrane

Mechanism  The peptide inhibits HIV fusion by binding to the hydrophobic grooves on the N-terminal heptad repeat (NHR) trimer and blocking six-helix-bundle (6-HB) formation.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00518

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C169H277N39O57S

Absent amino acids  CFGHPRW

Common amino acids  L

Mass  3799.35

Pl  4.08

Basic residues  2

Acidic residues  6

Hydrophobic residues  13

Net charge  -4

Boman Index  -3660

Hydrophobicity  -0.91

Aliphatic Index  130

Half Life 

  •     Mammalian:1 hour
  •     Yeast:30 min
  •     E.coli:>10 hour

Extinction Coefficient cystines  2980

Absorbance 280nm  93.13

Polar residues  9



Literature Information


Literature 1

Title   Creating an Artificial Tail Anchor as a Novel Strategy To Enhance the Potency of Peptide-Based HIV Fusion Inhibitors.

Pubmed ID   27795416

Reference   J Virol. 2016 Dec 16;91(1):e01445-16.

Author   Su S, Zhu Y, Ye S, Qi Q, Xia S, Ma Z, Yu F, Wang Q, Zhang R, Jiang S, Lu L. 

DOI   10.1128/JVI.01445-16