General Information


DRAVP ID  DRAVPe00575

Peptide Name   FP4(FCoV Sgp (1338-1380))

Sequence  FNATYLNLTGEIDDLEFRSEKLHNTTVELAILIDNINNTLVNL

Sequence Length  43

UniProt ID  P10033 

Source  Synthetic construct(derived from S protein of FCoV)



Activity Information


Target Organism  FCoV

Assay  plaque reduction assay

Activity 

  • [Ref.24312629]feline coronavirus(FCoV):inhibition of virus replication in Fcwf-4 cells(IC50=1.8 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.24312629]Fcwf-4 cell:CC50>200 μM.

Binding Target  membrane

Mechanism  The peptide can interfere the fusion of the viral envelope with host membrane and thus inhibits viral replication.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe00575

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C218H348N56O72

Absent amino acids  CMPQW

Common amino acids  L

Mass  4905.49

Pl  4.19

Basic residues  3

Acidic residues  7

Hydrophobic residues  18

Net charge  -4

Boman Index  -6376

Hydrophobicity  -1.4

Aliphatic Index  126.98

Half Life 

  •     Mammalian:1.1 hour
  •     Yeast:3 min
  •     E.coli:2 min

Extinction Coefficient cystines  1490

Absorbance 280nm  35.48

Polar residues  15



Literature Information


Literature 1

Title   Peptides corresponding to the predicted heptad repeat 2 domain of the feline coronavirus spike protein are potent inhibitors of viral infection.

Pubmed ID   24312629

Reference    PLoS One. 2013 Dec 3;8(12):e82081.

Author   Liu IJ, Tsai WT, Hsieh LE, Chueh LL.

DOI   10.1371/journal.pone.0082081