General Information

General Information

DRAVP ID DRAVPe00696

Peptide Name Indolicidin (Cathelicidin-4)

Sequence ILPWKWPWWPWRR

Sequence Length 13

UniProt ID P33046 A3KN14

Taxon ID None

Source Bos taurus (Bovine)

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not available

Activity Information

Target Organism HSV,HIV

Assay HIV-1 integrase inhibition assay

Activity

[Ref.15081088]Herpes simplex virus type 1 (HSV-1):inhibition of HSV-1 infection in Vero cells(EC50=28.68 μM);
Herpes simplex virus type 2 (HSV-2):inhibition of HSV-2 infection in Vero cells(EC50=5.00 μM).
[Ref.15482931]HIV-1:Inhibition of 3′-processing catalyzed by integrase(IC50=60 µM);inhibition of strand transfer catalyzed by integrase(IC50=57 µM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.15081088]Vero cells:CC50>100 μM.

Binding Target HIV Integrase

Mechanism The HIV-1 integrase plays a crucial role in the HIV-1 virus replication cycle. The peptide can inhibit the activity of integrase and thus inhibit virus replication.

Structure Information

PDB ID 1G8C 1G89

Predicted Structure Download DRAVPe00696

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidtion

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C100H131N25O14

Absent amino acids ACDEFGHMNQSTVY

Common amino acids W

Mass 1907.3

Pl 12.01

Basic residues 3

Acidic residues 0

Hydrophobic residues 7

Net charge 3

Boman Index -1390

Hydrophobicity -106.92

Aliphatic Index 60

Half Life

Mammalian:20 hour
Yeast:30 min
E.coli:>10 hour

Extinction Coefficient cystines 27500

Absorbance 280nm 2291.67

Polar residues 0

Literature Information

Literature 1

Title Antiviral activity of antimicrobial cationic peptides against Junin virus and herpes simplex virus.

Pubmed ID 15081088

Reference Int J Antimicrob Agents. 2004 Apr;23(4):382-9.

Author Albiol Matanic VC, Castilla V.

DOI 10.1016/j.ijantimicag.2003.07.022

Literature 2

Title Synthesis and HIV-1 integrase inhibitory activity of dimeric and tetrameric analogs of indolicidin.

Pubmed ID 15482931

Reference Bioorg Med Chem Lett. 2004 Nov 15;14(22):5595-8.

Author Krajewski K, Marchand C, Long YQ, Pommier Y, Roller PP.

DOI 10.1016/j.bmcl.2004.08.061