General Information

General Information

DRAVP ID DRAVPe00750

Peptide Name IIQ

Sequence IEEIQKKIEEIQKKIEEIQKKIEEIQKKIEEIQKKXX

Sequence Length 37

UniProt ID No entry found

Taxon ID None

Source Synthetic construct

Validation Experimentally Validated

Origin Information

Gene Name/ID Not Available

GenBank Not Available

Amino Acid position Not Available

Domain Accession ID Not Available

Nucleotide sequence ID Not Available

Molecular Type Not Available

Chromosomal Position Not Available

Activity Information

Target Organism MERS-CoV,H3N2,H1N1,HIV,EboV

Assay MERS-CoV S Protein-Mediated Cell–Cell Fusion Assay

Activity

[Ref.30192544]MERS-CoV:inhibition of cell-cell fusion between 293T / MERS / EGPF cells and Huh-7 cells(EC50=0.11±0.02 μM);inhibition of pseudovirus infection in Huh-7 cells(IC50=0.13±0.1 μM);
A/Puerto Rico/8/34 (H1N1):inhibition of virus infection in MDCK cells(EC50=1.73±0.81 μM);
A/Hong Kong/8/68 (H3N2):inhibition of virus infection in MDCK cells(EC50=0.70±0.09 μM);
HIV-1 inhibition of cell-cell fusion in TZM-bl cells(EC50=3.63 ±0.54 μM);
Ebola virus (EboV):inhibition of pseudovirus infection in TZM-bl cells(EC50=1.02 ± 0.54 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.30192544]Huh-7 cell:CC50>100 μM;
MDCK cell:CC50>100 μM;
TZM-bl cell:CC50>100 μM.

Binding Target membrane

Mechanism The peptide can interact with both MERS-CoV and IAV NHR trimeric coiled coils to prevent virus–cell membrane fusion.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Acetylation

C-terminal Modification Amidation

Other Modification The 'X' at 36 indicates β-alanine,the 'X' at 37 indicates Lys-C16(palmitic acid).

Stereochemistry L

Physicochemical Information

Formula C195H338N50O59

Absent amino acids ACDFGHLMNPRSTVWY

Common amino acids EIK

Mass 4585.82

Pl 6.49

Basic residues 10

Acidic residues 10

Hydrophobic residues 10

Net charge 0

Boman Index -10210

Hydrophobicity -125.68

Aliphatic Index 105.41

Half Life

Mammalian:20 hour
Yeast:30 min
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 0

Literature Information

Literature 1

Title De Novo Design of α-Helical Lipopeptides Targeting Viral Fusion Proteins: A Promising Strategy for Relatively Broad-Spectrum Antiviral Drug Discovery.

Pubmed ID 30192544

Reference J Med Chem. 2018 Oct 11;61(19):8734-8745.

Author Wang C, Zhao L, Xia S, Zhang T, Cao R, Liang G, Li Y, Meng G, Wang W, Shi W, Zhong W, Jiang S, Liu K.

DOI 10.1021/acs.jmedchem.8b00890