General Information

General Information

DRAVP ID DRAVPe00750

Peptide Name IIQ

Sequence IEEIQKKIEEIQKKIEEIQKKIEEIQKKIEEIQKKXX

Sequence Length 37

UniProt ID No entry found

Taxon ID

Source Synthetic construct

Validation

Origin Information

Gene Name/ID

GenBank

Amino Acid position

Domain Accession ID

Nucleotide sequence ID

Molecular Type

Chromosomal Position

Activity Information

Target Organism MERS-CoV,H3N2,H1N1,HIV,EboV

Assay MERS-CoV S Protein-Mediated Cell–Cell Fusion Assay

Activity

[Ref.30192544]MERS-CoV:inhibition of cell-cell fusion between 293T / MERS / EGPF cells and Huh-7 cells(EC50=0.11±0.02 μM);inhibition of pseudovirus infection in Huh-7 cells(IC50=0.13±0.1 μM);
A/Puerto Rico/8/34 (H1N1):inhibition of virus infection in MDCK cells(EC50=1.73±0.81 μM);
A/Hong Kong/8/68 (H3N2):inhibition of virus infection in MDCK cells(EC50=0.70±0.09 μM);
HIV-1 inhibition of cell-cell fusion in TZM-bl cells(EC50=3.63 ±0.54 μM);
Ebola virus (EboV):inhibition of pseudovirus infection in TZM-bl cells(EC50=1.02 ± 0.54 μM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.30192544]Huh-7 cell:CC50>100 μM;
MDCK cell:CC50>100 μM;
TZM-bl cell:CC50>100 μM.

Binding Target membrane

Mechanism The peptide can interact with both MERS-CoV and IAV NHR trimeric coiled coils to prevent virus–cell membrane fusion.

Structure Information

PDB ID None

Predicted Structure Download No predicted structure available

Linear/Cyclic Linear

N-terminal Modification Acetylation

C-terminal Modification Amidation

Other Modification The 'X' at 36 indicates β-alanine,the 'X' at 37 indicates Lys-C16(palmitic acid).

Stereochemistry L

Physicochemical Information

Formula C195H338N50O59

Absent amino acids ACDFGHLMNPRSTVWY

Common amino acids EIK

Mass 4585.82

Pl 6.49

Basic residues 10

Acidic residues 10

Hydrophobic residues 10

Net charge 0

Boman Index -10210

Hydrophobicity -125.68

Aliphatic Index 105.41

Half Life

Mammalian:20 hour
Yeast:30 min
E.coli:>10 hour

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 0

Literature Information

Literature 1

Title De Novo Design of α-Helical Lipopeptides Targeting Viral Fusion Proteins: A Promising Strategy for Relatively Broad-Spectrum Antiviral Drug Discovery.

Pubmed ID 30192544

Reference J Med Chem. 2018 Oct 11;61(19):8734-8745.

Author Wang C, Zhao L, Xia S, Zhang T, Cao R, Liang G, Li Y, Meng G, Wang W, Shi W, Zhong W, Jiang S, Liu K.

DOI 10.1021/acs.jmedchem.8b00890