General Information


DRAVP ID  DRAVPe01106

Peptide Name   33[11S](derived from HCV C5A)

Sequence  SWLRDIWDWISEVLSDFK

Sequence Length  18

UniProt ID  P26664 

Source  Synthetic construct(derived from HCV non-structural protein 5A)



Activity Information


Target Organism  HCV

Assay  RT-QPCR assay

Activity 

  • [Ref.18287023]Hepatitis C virus(HCV):inhibition of HCV infection in Huh-7.5.1 cells(IC50=13.5 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  membrane

Mechanism  The peptide destabilizes viral structural integrity and has viral membranolytic activity.It interacts with the viral membrane to disrupt its integrity, release viral capsids, and expose the viral genome to exonucleases for degradation.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01106

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C109H155N25O30

Absent amino acids  ACGHMNPQTY

Common amino acids  DSW

Mass  2295.58

Pl  4.23

Basic residues  2

Acidic residues  4

Hydrophobic residues  9

Net charge  -2

Boman Index  -2995

Hydrophobicity  -21.67

Aliphatic Index  102.78

Half Life 

  •     Mammalian:1.9 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  16500

Absorbance 280nm  970.59

Polar residues  3



Literature Information


Literature 1

Title   A virocidal amphipathic {alpha}-helical peptide that inhibits hepatitis C virus infection in vitro.

Pubmed ID   18287023

Reference   Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3088-93.

Author   Cheng G, Montero A, Gastaminza P, Whitten-Bauer C, Wieland SF, Isogawa M, Fredericksen B, Selvarajah S, Gallay PA, Ghadiri MR, Chisari FV.

DOI   10.1073/pnas.0712380105