General Information

General Information

DRAVP ID DRAVPe01165

Peptide Name PB1-1-15 B

Sequence MNINPYPLFIDVPIQ

Sequence Length 15

UniProt ID Q6DNS6

Source Synthetic construct(derived from INFV A polymerase (PB1))

Activity Information

Target Organism Influenza A

Assay ELISA

Activity

[Ref.19841738]Influenza A(FluA):inhibition of PA-binding activity and virus replication(IC50>3000 nM);
Influenza A(FluA):inhibition of PA-binding activity and virus replication(IC50=45.0 ±12.5 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target PA subunit

Mechanism Influenza virus replication and transcription is performed in the nucleus by the heterotrimeric viral polymerase complex consisting of the PB1, PB2, and PA subunits.The peptide could bind with PA subunit thus inhibit the virus replication.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01165

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C84H128N18O22S

Absent amino acids ACEGHKRSTW

Common amino acids IP

Mass 1774.11

Pl 3.8

Basic residues 0

Acidic residues 1

Hydrophobic residues 6

Net charge -1

Boman Index 137

Hydrophobicity 40.67

Aliphatic Index 123.33

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 1490

Absorbance 280nm 106.43

Polar residues 3

Literature Information

Literature 1

Title Identification of a PA-binding peptide with inhibitory activity against influenza A and B virus replication.

Pubmed ID 19841738

Reference PLoS One. 2009 Oct 20;4(10):e7517.

Author Wunderlich K, Mayer D, Ranadheera C, Holler AS, Mänz B, Martin A, Chase G, Tegge W, Frank R, Kessler U, Schwemmle M.

DOI 10.1371/journal.pone.0007517