General Information


DRAVP ID  DRAVPe01168

Peptide Name   PB1-1-15 A D2N,V3I

Sequence  MNINPTLLFLKVPAQ

Sequence Length  15

UniProt ID  Q6DNS6 

Source  Synthetic construct(derived from INFV A polymerase (PB1))



Activity Information


Target Organism  Influenza A

Assay  ELISA

Activity 

  • [Ref.19841738]Influenza A(FluA):inhibition of PA-binding activity and virus replication(IC50=12.96 ±3.98 nM);
  • Influenza A(FluA):inhibition of PA-binding activity and virus replication(IC50>3000 nM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  PA subunit

Mechanism  Influenza virus replication and transcription is performed in the nucleus by the heterotrimeric viral polymerase complex consisting of the PB1, PB2, and PA subunits.The peptide could bind with PA subunit thus inhibit the virus replication.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01168

SequenceNo structure available

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C79H131N19O20S

Absent amino acids  CDEGHRSWY

Common amino acids  L

Mass  1699.08

Pl  8.5

Basic residues  1

Acidic residues  0

Hydrophobic residues  7

Net charge  1

Boman Index  392

Hydrophobicity  55.33

Aliphatic Index  130

Half Life 

  •     Mammalian:30 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  3



Literature Information


Literature 1

Title   Identification of a PA-binding peptide with inhibitory activity against influenza A and B virus replication. 

Pubmed ID   19841738

Reference   PLoS One. 2009 Oct 20;4(10):e7517.

Author   Wunderlich K, Mayer D, Ranadheera C, Holler AS, Mänz B, Martin A, Chase G, Tegge W, Frank R, Kessler U, Schwemmle M.

DOI   10.1371/journal.pone.0007517