General Information

General Information

DRAVP ID DRAVPe01169

Peptide Name PB1-1-15 A T6Y,L7F

Sequence MDVNPYFLFLKVPAQ

Sequence Length 15

UniProt ID Q6DNS6

Source Synthetic construct(derived from INFV A polymerase (PB1))

Activity Information

Target Organism Influenza A

Assay ELISA

Activity

[Ref.19841738]Influenza A(FluA):inhibition of PA-binding activity and virus replication(IC50=7.51 ±0.71 nM);
Influenza A(FluA):inhibition of PA-binding activity and virus replication(IC50=345.0 ±81.5 nM).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity No cytotoxicity information found in the reference(s) presented

Binding Target PA subunit

Mechanism Influenza virus replication and transcription is performed in the nucleus by the heterotrimeric viral polymerase complex consisting of the PB1, PB2, and PA subunits.The peptide could bind with PA subunit thus inhibit the virus replication.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01169

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C86H128N18O21S

Absent amino acids CEGHIRSTW

Common amino acids FLPV

Mass 1782.13

Pl 5.59

Basic residues 1

Acidic residues 1

Hydrophobic residues 7

Net charge 0

Boman Index 145

Hydrophobicity 42.67

Aliphatic Index 97.33

Half Life

Mammalian:30 hour
Yeast:>20 hour
E.coli:>10 hour

Extinction Coefficient cystines 1490

Absorbance 280nm 106.43

Polar residues 2

Literature Information

Literature 1

Title Identification of a PA-binding peptide with inhibitory activity against influenza A and B virus replication.

Pubmed ID 19841738

Reference PLoS One. 2009 Oct 20;4(10):e7517.

Author Wunderlich K, Mayer D, Ranadheera C, Holler AS, Mänz B, Martin A, Chase G, Tegge W, Frank R, Kessler U, Schwemmle M.

DOI 10.1371/journal.pone.0007517