General Information

General Information

DRAVP ID DRAVPe01204

Peptide Name Mucroporin-M1

Sequence LFRLIKSLIKRLVSAFK

Sequence Length 17

UniProt ID B9UIY3

Source Synthetic construct(derived from Mucroporin)

Activity Information

Target Organism SARS-CoV,H5N1,MeV

Assay Plaque formation assay

Activity

[Ref.21620914]measles virus (MeV):inhibition of MeV infection in Vero cells(EC50=7.15 μg/ml (3.52 μM));
SARS-CoV(pseudoviruse):inhibition of SARS-CoV infection in MDCK cells(EC50=14.46 μg/ml (7.12 μM));
H5N1(pseudovirus):inhibition of H5N1 infection in MDCK cells(EC50=2.10 μg/ml (1.03 μM)).

Hemolytic Activity No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity

[Ref.21620914]Vero cells:CC50=70.46μg/ ml(34.70μM);
HeLa-ACE2 cells:CC50=61.58 μg/ml (30.31 μM);
MDCK cells:CC50=83.35 μg/ml (41.03 μM).

Binding Target membrane

Mechanism Mucroporin-M1 may bind to the virus envelope by surface charge interactions and inhibits MeV, SARS-CoV and influenza H5N1 by direct virucidal action.

Structure Information

PDB ID None

Predicted Structure Download DRAVPe01204

Linear/Cyclic Linear

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Stereochemistry L

Physicochemical Information

Formula C98H170N26O20

Absent amino acids CDEGHMNPQTWY

Common amino acids L

Mass 2032.59

Pl 12.02

Basic residues 5

Acidic residues 0

Hydrophobic residues 10

Net charge 5

Boman Index -1196

Hydrophobicity 79.41

Aliphatic Index 160.59

Half Life

Mammalian:5.5 hour
Yeast:3 min
E.coli:2 min

Extinction Coefficient cystines 0

Absorbance 280nm 0

Polar residues 2

Literature Information

Literature 1

Title Virucidal activity of a scorpion venom peptide variant mucroporin-M1 against measles, SARS-CoV and influenza H5N1 viruses.

Pubmed ID 21620914

Reference Peptides. 2011 Jul;32(7):1518-25.

Author Li Q, Zhao Z, Zhou D, Chen Y, Hong W, Cao L, Yang J, Zhang Y, Shi W, Cao Z, Wu Y, Yan H, Li W.

DOI 10.1016/j.peptides.2011.05.015