DRAVP ID  DRAVPe01204

Peptide Name   Mucroporin-M1

Sequence  LFRLIKSLIKRLVSAFK

Sequence Length  17

UniProt ID  B9UIY3 

Taxon ID  None

Source  Synthetic construct(derived from Mucroporin)

Validation   Experimentally Validated

Gene Name/ID  Not Available

GenBank  Not Available

Amino Acid position  Not Available

Domain Accession ID  Not Available

Nucleotide sequence ID  Not Available

Molecular Type  Not Available

Chromosomal Position  Not available

Target Organism  SARS-CoV,H5N1,MeV

Assay  Plaque formation assay

Activity 

• [Ref.21620914]measles virus (MeV):inhibition of MeV infection in Vero cells(EC50=7.15 μg/ml (3.52 μM));
• SARS-CoV(pseudoviruse):inhibition of SARS-CoV infection in MDCK cells(EC50=14.46 μg/ml (7.12 μM));
• H5N1(pseudovirus):inhibition of H5N1 infection in MDCK cells(EC50=2.10 μg/ml (1.03 μM)).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.21620914]Vero cells:CC50=70.46μg/ ml(34.70μM);
• HeLa-ACE2 cells:CC50=61.58 μg/ml (30.31 μM);
• MDCK cells:CC50=83.35 μg/ml (41.03 μM).

Binding Target  membrane

Mechanism  Mucroporin-M1 may bind to the virus envelope by surface charge interactions and inhibits MeV, SARS-CoV and influenza H5N1 by direct virucidal action.

PDB ID  None

Predicted Structure Download  DRAVPe01204

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Stereochemistry  L

Literature 1

Title   Virucidal activity of a scorpion venom peptide variant mucroporin-M1 against measles, SARS-CoV and influenza H5N1 viruses. 

Pubmed ID   21620914

Reference   Peptides. 2011 Jul;32(7):1518-25.

Author   Li Q, Zhao Z, Zhou D, Chen Y, Hong W, Cao L, Yang J, Zhang Y, Shi W, Cao Z, Wu Y, Yan H, Li W. 

DOI   10.1016/j.peptides.2011.05.015