General Information


DRAVP ID  DRAVPe01288

Peptide Name   DV3(419-447)

Sequence  AWDFGSVGGVLNSLGKMVHQIFGSAYTAL

Sequence Length  29

UniProt ID  Q99D35 

Source  Synthetic construct(derived from DENV3 E protein stem)



Activity Information


Target Organism  DENV

Assay  Plaque assay

Activity 

  • [Ref.20881042]Dengue 1 virus(DENV1):inhibition of infection in BHK-21 cells(IC90<0.1 μM);
  • Dengue 2 virus(DENV2):inhibition of infection in BHK-21 cells(IC90=2 μM);
  • Dengue 3 virus(DENV3):inhibition of infection in BHK-21 cells(IC90=4 μM);
  • Dengue 4 virus(DENV4):inhibition of infection in BHK-21 cells(IC90=1.5 μM).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity  No cytotoxicity information found in the reference(s) presented

Binding Target  Not found

Mechanism  The peptide shows antiviral activity by inhibiting virus entry.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01288

Linear/Cyclic  Linear

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Stereochemistry  L



Physicochemical Information


Formula  C139H209N35O39S

Absent amino acids  CEPR

Common amino acids  G

Mass  3026.46

Pl  6.79

Basic residues  2

Acidic residues  1

Hydrophobic residues  13

Net charge  1

Boman Index  855

Hydrophobicity  56.9

Aliphatic Index  94.14

Half Life 

  •     Mammalian:4.4 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  6990

Absorbance 280nm  249.64

Polar residues  11



Literature Information


Literature 1

Title   Peptide inhibitors of flavivirus entry derived from the E protein stem.

Pubmed ID   20881042

Reference   J Virol. 2010 Dec;84(24):12549-54.

Author   Schmidt AG, Yang PL, Harrison SC.

DOI   10.1128/JVI.01440-10