DRAVP ID  DRAVPe01304

Peptide Name   HD5[E21R]

Sequence  ATCYCRTGRCATRESLSGVCRISGRLYRLCCR

Sequence Length  32

UniProt ID  Q01523 

Source  Synthetic construct(derived from human alpha defensin(HD5))

Target Organism  HSV,HIV

Assay  Plaque assay

Activity 

• [Ref.23269800]Herpes simplex virus2(HSV-2):Potential for CaSki cell protection from HSV-2 infection(61.5% ± 7.1% cell protection rates at 25 μg/ml,90.2% ± 4.9% cell protection at 50 μg/ml);neutralization activity against HSV-2 during the preinfection stage(IC50=25 μg/ml);
• HIV-1:inhibition of virus infection in JLTRG cells(IC50=3.53 μg/ ml).

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

• [Ref.23269800]No cytotoxicity against CaSki cells up to 100μg/ ml.

Binding Target  capsid protein gD

Mechanism  The interaction between peptide and viral capsid proteins may cause aggregation of virions to prevent viruses from normal binding to target cells. Another possibility is that the binding of HD5 derivatives to the virion envelope blocks the epitopes of interaction between viruses and cell receptors, which are necessary for viral adsorption or entry into host cells.

PDB ID  None

Predicted Structure Download  DRAVPe01304

Linear/Cyclic  Cyclic

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry  L

Literature 1

Title   Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites.

Pubmed ID   23269800

Reference   J Virol. 2013 Mar;87(5):2835-45. 

Author   Wang A, Chen F, Wang Y, Shen M, Xu Y, Hu J, Wang S, Geng F, Wang C, Ran X, Su Y, Cheng T, Wang J. 

DOI   10.1128/JVI.02209-12