General Information


DRAVP ID  DRAVPe01306

Peptide Name   HD5[T7R]

Sequence  ATCYCRRGRCATRESLSGVCEISGRLYRLCCR

Sequence Length  32

UniProt ID  Q01523 

Source  Synthetic construct(derived from human alpha defensin(HD5))



Activity Information


Target Organism  HSV

Assay  Plaque assay

Activity 

  • [Ref.23269800]Herpes simplex virus2(HSV-2):neutralization activity against HSV-2 during the preinfection stage(IC50=40 μg/ml);

Hemolytic Activity  No hemolysis information or data found in the reference(s) presented in this entry

Cytotoxicity 

  • [Ref.23269800]No cytotoxicity against CaSki cells up to 100μg/ ml.

Binding Target  capsid protein gD

Mechanism  The interaction between peptide and viral capsid proteins may cause aggregation of virions to prevent viruses from normal binding to target cells. Another possibility is that the binding of HD5 derivatives to the virion envelope blocks the epitopes of interaction between viruses and cell receptors, which are necessary for viral adsorption or entry into host cells.



Structure Information


PDB ID  None

Predicted Structure Download  DRAVPe01306

Linear/Cyclic  Cyclic

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Disulfide bonds between Cys3 and Cys31,Cys5 and Cys20,Cys10 and Cys30.

Stereochemistry  L



Physicochemical Information


Formula  C146H249N53O44S6

Absent amino acids  DFHKMNPQW

Common amino acids  R

Mass  3643.27

Pl  9.22

Basic residues  7

Acidic residues  2

Hydrophobic residues  7

Net charge  5

Boman Index  -9584

Hydrophobicity  -23.13

Aliphatic Index  64.06

Half Life 

  •     Mammalian:4.4 hour
  •     Yeast:>20 hour
  •     E.coli:>10 hour

Extinction Coefficient cystines  3355

Absorbance 280nm  108.23

Polar residues  16



Literature Information


Literature 1

Title   Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites.

Pubmed ID   23269800

Reference   J Virol. 2013 Mar;87(5):2835-45. 

Author   Wang A, Chen F, Wang Y, Shen M, Xu Y, Hu J, Wang S, Geng F, Wang C, Ran X, Su Y, Cheng T, Wang J. 

DOI   10.1128/JVI.02209-12